针刺对多发梗塞性痴呆大鼠海马NF-κB和IκB表达的影响  

Effect of acupuncture on expression of NF-κB and IκB in cerebral multi-infarction rats

作　　者：刘存志[1] 石广霞[1] 管莉萍[2] 李祖光[3] 杨肖敏[2] 

机构地区：[1]首都医科大学附属北京中医医院,北京100010 [2]天津中医药大学,天津300193 [3]天津中医药大学附属南开中医院,天津300102

出　　处：《中华中医药杂志》2011年第7期1618-1620,共3页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：教育部新世纪优秀人才支持计划(No.NCET-09-0007);北京市科技新星计划(No.2009B46)~~

摘　　要：目的:观察针刺对多发梗塞性痴呆大鼠海马NF-κB和IκB蛋白表达的影响,探讨针刺治疗多发梗塞性痴呆的作用机制。方法:通过栓子注入法制作多发梗塞性痴呆模型,造模成功大鼠随机分为模型组、针刺组和非穴组,并设正常组和假手术组。针刺处理3周后,运用免疫组化方法分析海马NF-κB和IκB的蛋白表达水平。结果:与正常组相比,模型组海马NF-κB及IκB蛋白表达显著升高。同时,针刺组大鼠海马NF-κB、IκB蛋白表达水平明显高于模型组。结论:针刺能够增强多发梗塞性痴呆大鼠海马NF-κB、IκB的蛋白表达水平,发挥神经保护作用,改善痴呆动物的学习记忆能力。Objective： To observe the influence of acupuncture on the protein expression of nuclear factor-κB（NF-κB） and inhibitor nuclear factor-kappa B（IκB） in hippocampus of multi-infarct dementia（MID） rats and explore the mechanism of acupuncture on MID.Method： The model of MID in rats was made by injecting homogeneous emboli into the internal carotid artery.The MID rats were randomly divided into model,acupuncture and sham-acupuncture groups.Meanwhile,normal and sham-operated rats were used as control.The protein expression levels of NF-κB and IκB in hippocampus of all rats were evaluated by immunohistochemistry method following a three-week period of acupunctural treatment.Result： A signi？cant increase in the protein expression of NF-κB was observed in hippocampus of rats in model group when compared with normal rats.Meanwhile,significant increases in the protein expression of NF-κB and IκB were found in hippocampus of rats in the acupuncture group when compared with the model rats.Conclusion： Acupuncture could exert neuroprotective effects and improve the abilities of learning and memory of MID rats by increasing the protein expression of NF-κB and IκB.

关 键 词：针刺 多发梗塞性痴呆 大鼠 核转录因子-ΚB 抑制核转录因子κB 

分 类 号：R245[医药卫生—针灸推拿学]