卵巢切除后鼠椎间盘结构及软骨P物质神经分布的观察  

Structural Changes and Localization of Substance P and Neurofilament Immunoreactive Fibers in Intervertebral Disc of Ovariectomied Rats

作　　者：胡侦明[1,2,3] 戴尅戎[1,2,3] 赵建平 

机构地区：[1]上海第二医科大学附属第九人民医院骨科 [2]昆明医学院第二附属医院骨科 [3]上海医科大学解剖教研室

出　　处：《中华骨科杂志》1999年第5期292-294,I001,共4页Chinese Journal of Orthopaedics

摘　　要：目的研究卵巢切除后鼠椎间盘结构及软骨Ｐ物质神经分布变化，探索骨质疏松性腰背部疼痛的原因。方法３月龄雌性ＳＤ大鼠６４只，分组行双侧卵巢切除（ＯＶＸ）及假手术（Ｓｈａｍ－Ｏ）；术后第１、３、５、７个月取Ｌ３－４、Ｌ４－５椎间盘。研究采用胶原ＶＧ特染，光、电镜观察及免疫组织化学ＰＡＰ等方法。结果Ｓｈａｍ－０组各阶段纤维环中胶原纤维结构排列规则，偶尔见到较小的串珠状ＳＰ阳性反应物。ＯＶＸ组纤维环中胶原结构排列紊乱、断裂，以后部改变较明显，软骨细胞退变。椎间盘软骨及周围组织可见较多的ＳＰ免疫反应阳性神经末梢。结论ＯＶＸ后椎间盘较早出现退行性改变，结构及功能的完整性破坏；ＳＰ的增加与局部炎症反应相关，可能与骨质疏松性腰背疼痛有关。Objective To study the correlations of structural changes and localization of substance P neurofilament fibers in ovariectomied rats and to understand the basis of osteoporotic back pain Methods Sixty four 3 months old female SD rats were divided into two groups, one underwent ovariectomy(OVX) and the other shamoperation (Sham-O) Sixteen animals in earch batch were sacrificed at 1, 3, 5 , 7th month to procure the L3-4, L4-5 intervertebral disc specimens for the examinations by means of collagen VG stain, microscopy, scanning, transmission electron microscopy and the immunohistochemical PAP of disc cartilage Results Great changes were seen in annulus fibrosus and nucleus of intervertebral disc such as breaking or rupture of collagen fiberlining, particularlly in the posterior aspect of the annulus fibrosus, chondrocytes showed degenerative changes as compared with Sham-O control group And the existence of SP immunoreactive nerve fibers in disc cartilage and annulus fibrosus from the first month, which existed as neurofilament with the orlentation following collagen fibers SP positive nerve fibers were present and increasing in quantity with aging through 7th month Also SP nerve fibers were present in disc surrounding tissue and related to the local inflammation Conclusions The integration of structure and function were broken and degeneration of intervertebral disc took place early after OVX; increase of SP was possibly related with local inflammatory process as well as with lower back pain in estrogen deficiency osteoporosis

关 键 词：卵巢切除术 椎间盘 胶原 P4物质 大鼠 软骨 

分 类 号：R713.606[医药卫生—妇产科学] R681[医药卫生—临床医学]