离体异位子宫内膜细胞中单核细胞趋化蛋白-1的测定  

Determination of Monocyte Chemotactic Protein 1 in Cultured Endometriotic Cells

作　　者：高颖[1] 罗丽兰 何福仙 

机构地区：[1]同济医科大学附属协和医院妇产科,武汉430022

出　　处：《中华妇产科杂志》1999年第10期612-614,I015,共4页Chinese Journal of Obstetrics and Gynecology

摘　　要：目的　探讨异位子宫内膜细胞产生单核细胞趋化蛋白１（ＭＣＰ１）的临床意义。方法　收集１５ 例子宫内膜异位症患者的异位内膜组织进行体外细胞培养，分别加入纯培养液（ 对照组） 、含白细胞介素１β（ＩＬ１β）２ ｎｇ／ｍｌ（ＩＬ１β诱导组）及肿瘤坏死因子α（ＴＮＦα）２０ ｍｇ／ｍｌ（ＴＮＦα诱导组）的培养液培养４ 小时，应用斑点杂交、免疫组织化学及夹心酶联免疫吸附试验检测各组异位细胞中ＭＣＰ１ｍＲＮＡ蛋白的表达水平及其培养上清中ＭＣＰ１ 的含量。结果　与对照组相比，异位内膜细胞经ＩＬ１β及ＴＮＦα作用后表达的ＭＣＰ１ ｍＲＮＡ和蛋白水平显著增加（ Ｐ＜ ０．０１ ，Ｐ＜０．０５）；培养上清中ＭＣＰ１ 的含量也显著增加（Ｐ＜０ ．０１ ，Ｐ＜ ０．０５）。结论　ＩＬ１β及ＴＮＦα可刺激异位内膜细胞中ＭＣＰ１ 的表达显著增加，这可能和子宫内膜异位症的病变发展有关。Objective To investigate the clinical significance of monocyte chemotactic protein 1 (MCP 1) produced by endometriotic tissues. Methods Expressions of MCP 1 mRNA and MCP 1 protein were determined by dot blot analysis, immunohistochemical method [streptavidin biotin peroxidase complex (SABC)] and enzyme linked immunosorbent assay (ELISA) methods in cultured endometriotic cells with median (controls) or with interleukin 1β(IL 1β)2 ng/ml (IL 1β group), and tumor necrosis factor α(TNF α)20 mg/ml(TNF α group) respectively. The endometriotic tissues were sourced from 15 patients with endometriosis. Meanwhile, the MCP 1 protein content of cultured supernatent was also measured. Results After exposured to IL 1β or TNF α, the expression of MCP 1 mRNA and MEP 1 protein in the endometriotic cells were significantly higher than that in the control group ( P <0.01, P <0.05 respectively); so was the MCP 1 protien content of supernatent. Conclusions IL 1 β and TNF α can up regulate the expression of MCP 1 in endometriotic cells, which may be related to the development of emdometriosis.

关 键 词：子宫内膜异位 趋化蛋白-1 MCP-1 单核细胞 

分 类 号：R711.71[医药卫生—妇产科学]