两种方法检测乙肝血清学标志物的比较分析  

作　　者：徐红珍[1] 王露[1] 

机构地区：[1]姜堰市人民医院检验科,225500

出　　处：《当代医学》2011年第19期29-31,共3页Contemporary Medicine

摘　　要：目的探讨用酶联免疫吸附实验法(ELISA)和时间分辨荧光免疫法(TRFIA)检测乙肝两对半的差异。方法分别用ELISA法和TRFIA法对102例标本进行检测,分析两种方法的检测结果及其检出模式。研究两种方法检测乙肝表面抗原(HBsAg)的线性范围和最低检测浓度。结果两种方法检测乙肝两对半的HBsAg、表面抗体(HBsAb)和e抗原(HBeAg)无显著差异(P>0.05),e抗体(HBeAb)和核心抗体(HBcAb)有显著差异(P<0.05)。2种方法检测135、13模式,其结果一致,检测其它几种常见模式及少见模式,结果有差别。两法相比,TRFIA法检测HBsAg具有相当宽的线性范围和最低检测浓度。结论 TRFIA法定量检测乙肝两对半具有灵敏度高、特异性强、稳定性好等特点,是一种理想的乙肝两对半定量检测方法。Objective To explore the difference of ELISA and TRFIA while detecting five markers of Hepatitis B virus. Methods 102 samples were measured by ELISA and TRFIA to study their positive rates,then their detecting model were researched,their repetitiveness was checked. The linearity range and stability of two methods was evaluated. Results There are no big differences while HBsAg,HBsAb and HBeAg are detected with the two methods-ELISA and TRFIA（P〈0.05） . But there are significant differences while HBeAb and HBcAb are detected with the two methods（P〈0.05） . Their results are the same while 135 and 13 modes are detected. But their results are different while other common modes and seldom modes are detected. The repetitiveness of TRFIA is better than that of ELISA. There are the wider linearity range and the lowest limit of detection of TRFIA.Conclusion TRFIA is a sensitive,specific,stable and quantitative assay. So it is an ideal method to detect five markers of Hepatitis B virus.

关 键 词：酶联免疫吸附实验 时间分辨荧光免疫 乙肝两对半 

分 类 号：R446.6[医药卫生—诊断学]