大黄酸-雌酮对去卵巢大鼠骨质量的保护作用  

Protective effect of rhein-estrone on the bone quality of ovariectomized rats

作　　者：唐存贵[1,2] 李灵芝[1,3] 金鑫[1] 王越[1,3] 崔颖[1,3] 张永亮[1,3] 

机构地区：[1]武警医学院,天津300162 [2]武警广西总队医院 [3]天津市职业与环境危害生物标志物重点实验室

出　　处：《中国骨质疏松杂志》2011年第6期484-488,525,共6页Chinese Journal of Osteoporosis

基　　金：天津市自然科学基金课题(033609811);中国博士后科学基金面上项目(20080441340)

摘　　要：目的观察新型雌激素衍生物—大黄酸-雌酮对去卵巢大鼠骨质量的保护作用及其对子宫的雌激素样作用,以评价大黄酸-哌嗪雌酚酮的抗骨质疏松活性。方法 6月龄Wistar雌性未孕大鼠48只,分为假手术组、去卵巢模型组、雌酚酮组、大黄酸-雌酮高、中、低3个剂量组,除假手术组外其余各组均行双侧卵巢切除,给药24 w后,测定大鼠全身及脊柱骨密度、骨矿盐含量、股骨生物力学及骨羟脯氨酸、骨钙含量和观察大鼠股骨远端和子宫的形态学变化。结果大黄酸-雌酮3个剂量组大鼠全身及脊柱骨密度、骨矿物含量、最大压缩载荷、最大压缩应力、骨羟脯氨酸含量及股骨远端骺板下小梁骨体积均明显高于模型组(P<0.05或P<0.01);除骨体积少于雌酚酮组外,其余指标与雌酚酮组无明显差异;3组子宫重量、直径和内膜厚度明显小于雌酚酮组和假手术组,与模型组无明显差异。结论大黄酸-雌酮在本实验剂量范围内与雌酚酮一样能显著改善去卵巢大鼠骨质量,而对子宫的刺激作用远远低于雌酚酮。Objective To observe the protective effect of rhein-estrone, a novel hybrid compound of estrone and rhein, on the bone quality of ovariectomized （OVX） rats and its estrogen-like effect on the uterine, for evaluating its anti-osteoporosis activity. Methods Forty-eight 6-month-old wistar unpregnant female rats were divided into sham operating group, OVX model group, estrone group, rhein-estrone groups with high-, moderate-, or low-dose of rhein-estrone. Rats were ovariectomized in all groups except in sham operating group. After a 24-week drug administration, rat bone mineral density of the whole body and the spine, bone mineral content （BMC）, biomechanic proporties of the femur, contents of hydroxyproline and calcium, morphology of the distal femur and the uterus were examined. Results Compared to ovariectomized model rats, a significant increase in BMD of total body and the spine, BMC, maximum compress load, maximum compress stress, contents of hydroxyproline, and bone volume of the trabeeular bone were observed in rats treated with three dosages of rhein-estrone （ P 〈 0. 05 or P 〈 0. 01 ）. Except for low bone volume compared to that in estrone group, other parameters were not defferent. The weight, diameter and endometrium thickness of the uterus in rats treated with three dosages of rhein-estrone were significantly less than those in estrone and sham groups, but were no difference compared to those in OVX model group. Conclusion Rheinestrone significantly improved bone quality of OVX rats in the range of experimental dosages, as did by estrone. But its stimulating effect on the uterus was far less than estrone＇s.

关 键 词：大黄酸 雌酚酮 骨质疏松 骨质量 

分 类 号：R965[医药卫生—药理学]