HBeAg能上调新生儿脐带血中调节性T细胞的表达  

HBeAg can up regulate regulatory T cell in cord blood

作　　者：许晓梅[1] 施可庆[1] 刘建[2] 孙江川[2] 刘菊莲[3] 冯丽娟[3] 张大志[1] 

机构地区：[1]重庆医科大学附属第二医院感染科,重庆400010 [2]重庆医科大学附属第二医院产科,重庆400010 [3]重庆市妇幼保健院产科,重庆400013

出　　处：《重庆医科大学学报》2011年第5期550-553,共4页Journal of Chongqing Medical University

基　　金：国家自然科学基金资助项目(编号:30872250)

摘　　要：目的:观察HBsAg阳性产妇新生儿脐带血CD4+CD25+CD127low/-调节性T细胞(RegulatoryTcell,Treg)的数量,探讨HBeAg在新生儿HBV持续性感染中的作用。方法:共收集63例HBsAg阳性产妇新生儿脐带血,按照产妇产前HBeAg状态分为A组33例HBeAg阳性,B组30例HBeAg阴性,C组22例HBsAg阴性产妇做为对照组。利用三色流式分析法分析新生儿脐带血单个核细胞(Cord blood monocytes,CBMCs)中的Treg的频数。并检测产妇及其脐带血中的HBV血清标志物和HBV DNA水平。结果:A组33例其中有24例脐带血HBeAg阳性;B组和C组脐带血HBeAg均为阴性。所有HBsAg阳性产妇新生儿脐带血HBVDNA均为阴性。新生儿脐带血HBeAg水平与产妇外周血HBeAg水平无关(P>0.05)。与B组和C组相比较,A组新生儿脐带血中的Treg百分比明显升高,差异有统计学意义(P<0.05)。而B组和C组之间差异无统计学意义(P>0.05)。A组脐带血Treg百分比与产妇HBVDNA、HBeAg及脐带血HBeAg水平无关(P>0.05)。结论:HBeAg可以通过胎盘到达胎儿体内,可能通过上调Treg的比例,使HBeAg阳性产妇新生儿在宫内对HBV产生免疫耐受。Objective：To detect the frequency of CD4＋CD25＋CD127low/-regulatory T cell（Treg）in cord blood born from HBsAg positive mother and to determine the relationship between mother＇s HBeAg and HBV persistent infection after the children were born.Methods：According to mother＇s HBeAg status,63 cord blood of neonates delivered by HBsAg positive mothers were divided into group A（HBeAg positive）and group B（HBeAg negative）.22 cases of cord blood of neonates from HBsAg negative mother were served as group C（healthy controls）.The frequency of Treg was analyzed by three-color immunofluorescence flow cytometry.HBV serum markers and HBVDNA level both in cord blood and mother＇s peripheral blood were detected.Results：In group A,24 of 33 cord blood of neonates were HBeAg positive,but none of them were HBeAg positive in group B and group C.All of the cord blood were HBVDNA negative.There was no relationship about the HBeAg quantity between mother＇s peripheral blood and cord blood（P〉0.05）.Compared with group B and group C,the percentage of Treg significantly increased in group A（P〈0.05）,but the number was not dramatically different between group B and group C（P〉0.05）.The frequency of Treg did not relate to HBVDNA level and HBeAg quantity either in mother＇s peripheral blood or in cord blood.Conclusion：HBeAg may have substantial impact on the development of fetal immune system and bring on their immunotolerance to HBV by upregulating Treg in cord blood.

关 键 词：HBSAG HBEAG 脐带血 调节性T细胞 免疫耐受 

分 类 号：R512.6[医药卫生—内科学]