血清胆汁酸代谢轮廓分析在妊娠肝内胆汁淤积症诊断中的应用  

Intrahepatic cholestasis of pregnancy diagnosis based on metabolic profiling of serum bile acid

作　　者：田刚[1] 甄乾娜[1] 班艳娜[1] 唐秀芳[1] 丁敏[1] 

机构地区：[1]重庆医科大学检验系临床检验诊断学教育部重点实验室,重庆400016

出　　处：《重庆医科大学学报》2011年第5期586-588,共3页Journal of Chongqing Medical University

基　　金：国家自然科学基金资助项目(编号:20475069)

摘　　要：目的:探讨血清胆汁酸代谢轮廓分析在妊娠肝内胆汁淤积症(Intrahepaticcholestasisofpregnancy,ICP)诊断中的作用。方法:应用柱前衍生HPLC-荧光检测法测定ICP患者,乙肝孕妇和正常孕妇(各11例)血清胆汁酸谱(5种游离型和4种甘氨结合型),结合主成分分析法(Principal component analysis,PCA)和偏最小二乘判别分析法(Partial least-squares discriminantanalysis,PLS-DA)对血清胆汁酸谱进行分析。结果:血清胆汁酸代谢轮廓分析可区分ICP患者,乙肝孕妇和正常孕妇,其模型的正确诊断率和预测能力分别为97.0%和75.3%。结论:血清胆汁酸代谢轮廓分析有助于ICP的诊断和鉴别诊断。Objective：To study the feasibility of metabolic profiling of serum bile acid in intrahepatic cholestasis of pregnancy（ICP）diagnoses.Methods：High performance liquid chromatography（HPLC）with post column derivatisation and fluorescence detection were used to determinate 5 free and 4 glycine-conjugated serum bile acids in 11 healthy pregnant women,11 patients with ICP and 11 patients with hepatitis B.Data sets were analyzed by principal component analysis（PCA）and partial least-squares discriminant analysis（PLS-DA）.Results：PCA and PLS-DA models showed a clear separation among ICP group,hepatitis B group and healthy controls,and PLS-DA model provided an accuracy of 97.0% for the diagnosis of diseases and a prediction power of 75.3%.Conclusion：Metabolic profiling analysis of serum bile acid is useful for diagnosis of ICP.

关 键 词：胆汁酸 代谢轮廓分析 血清 妊娠肝内胆汁淤积症 乙型肝炎 

分 类 号：R714.255[医药卫生—妇产科学]