补肾活血汤对制动应激雌性大鼠海马神经生化的影响  

Effects of Bushen Huoxue Decoction on Neurobiochemical Markers in the Hippocampus of Female Rats with Repeated Immobilization Stress

作　　者：王佩娟[1,2] 彭蕴茹[3] 罗宇慧[3] 孔铭[4] 

机构地区：[1]江苏省中医药研究院妇产科,南京210028 [2]江苏省中西医结合医院妇产科,南京210028 [3]江苏省中医药研究院药理研究院,南京210028 [4]江苏省中医药研究院中药分析实验室,南京210028

出　　处：《中国中西医结合杂志》2011年第6期794-798,共5页Chinese Journal of Integrated Traditional and Western Medicine

基　　金：江苏省自然科学基金资助项目(No.BK2007174);康缘中医药科技创新基金资助项目(No.HZ0811KY)

摘　　要：目的探讨补肾活血汤对制动应激雌性大鼠海马神经生化的影响。方法 60只雌性大鼠随机分为正常组、模型组、六味地黄丸组(3.3 g生药/kg)、补肾活血汤高(8 g生药/kg)、中(4 g生药/kg)、低剂量组(2 g生药/kg),每组10只,采用重复制动应激法造成大鼠慢性心理应激模型,造模同时灌胃给药,每天1次,连续20天。给药结束后以高效液相色谱法检测海马组织神经介质水平的变化,并以Western blot法检测海马中脑源性神经营养因子(brain-derived neurotrophic factor,BDNF)及其受体水平的变化,观察补肾活血汤对制动应激大鼠海马神经生化的影响。结果正常大鼠海马组织中神经递质谷氨酸(Glu)、γ氨基丁酸(γ-am inobutyric acid,GABA)含量及BD-NF灰度值分别为(1 280.0±258.3)ng/mg、(588.3±115.1)ng/mg、13.26±2.57,与正常组比较,模型组大鼠海马组织Glu含量[(1 016.9±215.9)ng/mg]、GABA含量[(485.1±71.0)ng/mg]及BDNF灰度值(7.23±0.61)均明显降低(P<0.05),而补肾活血汤高、中、低剂量组Glu含量分别为(1 459.1±413.5)、(1 894.7±542.8)、(1 373.3±345.7)ng/mg,GABA含量分别为(631.6±161.4)、(899.1±262.1)、(656.4±140.8)ng/mg,BDNF灰度值分别为16.57±1.52、29.85±1.37、24.44±3.81,均较模型组显著升高(P<0.05,P<0.01),六味地黄丸组Glu含量[(1216.5±193.8)ng/mg]明显高于模型组(P<0.05)。结论补肾活血汤对制动应激雌性大鼠海马组织中相关神经介质水平和BDNF表达水平有明显的调节作用。Objective To study the effect of Bushen Huoxue Decoction（BHD） on neurobiochemical markers in the hippocampus of female rats with repeated immobilization stress.Methods Sixty female rats were randomly divided into the normal group,the model group,the positive control group（treated with Liuwei Dihuang Pill at the dose of 3.3 g crude drug/kg）,and the high,middle,and low BHD treated groups（at the dose of 8,4,2 g crude drug/kg）,ten in each group.Chronic psychological stress was induced using repeated immobilization stress in rats.Medication was conducted by gastrogavage while modeling once a day for twenty successive days.The hippocampal neurohumoral levels were detected with high-performance liquid chromatography.The expression levels of BDNF and its receptor in the hippocampus were detected by Western blot.Effect of BHD on neurobiochemical markers in the hippocampus of rats with repeated immobilization stress was observed.Results The levels of Glu,GABA,and BDNF in the hippocampus of the normal group were 1 280.0±258.3 ng/mg,588.3±115.1 ng/mg,and 13.26±2.57 gray value,respectively.But the hippocampal neurohumoral levels and the expression of BDNF in the model group obviously decreased when compared with the normal group,being 1 016.9±215.9 ng/mg,485.1±71.0 ng/mg,and 7.23±0.61 gray value,respectively.The levels of Glu（ng/mg） in hippocampus of the three BHD treated groups were 1 459.1±413.5,1 894.7±542.8,and 1 373.3±345.7,respectively.GABA levels（ng/mg） in the hippocampus were 631.6±161.4,899.1±262.1,and 656.4±140.8,respectively.BDNF levels（gray value） were 16.57±1.52,29.85±1.37,and 24.44±3.81,respectively,significantly higher than that of the model group（P0.05,P0.01）.The level of Glu in the positive control group（1 216.5±193.8 ng/mg） was significantly higher than that of model group（P0.05）.Conclusion BHD showed significant accommodation on the hippocampal neurohumoral levels and the expression of BDNF in the female rats with repeated immobilization stress.

关 键 词：补肾活血汤 制动应激 海马 

分 类 号：R285.5[医药卫生—中药学]