黄芪甲苷对内皮祖细胞氧化损伤的影响  

Effect of Astragaloside against the Oxidative Damage on Endothelial Progenitor Cells

作　　者：季亢挺[1] 唐疾飞[1] 柴俊德[1] 陈邢玉[1] 林加锋[1] 杨鹏麟[1] 

机构地区：[1]温州医学院附属第二医院心内科,浙江325000

出　　处：《中国中西医结合杂志》2011年第6期807-810,共4页Chinese Journal of Integrated Traditional and Western Medicine

基　　金：浙江省教育厅课题(No.Y201017426);浙江省卫生厅基金项目(No.2007A142);温州市科技局项目(No.H20080027)

摘　　要：目的观察黄芪甲苷对氧化低密度脂蛋白(oxidative low-density lipoprotein,Ox-LDL)介导的内皮祖细胞(endothelial progenitor cells,EPCs)氧化损伤的影响。方法密度梯度离心法获取外周血单个核细胞,通过流式细胞仪鉴定EPCs。培养7天后,收集贴壁细胞并随机分为正常对照组,Ox-LDL组(模型组,100μg/mL)及黄芪甲苷低、中、高剂量组(Ox-LDL 100μg/mL加黄芪甲苷2、10、50μg/mL),24 h后分别采用MTT比色法、黏附能力检测测定EPCs的增殖能力、黏附能力,并取各组细胞上清液检测超氧化物歧化酶(SOD)、丙二醛(MDA)水平。结果 Ox-LDL损伤后,外周血EPCs的增殖能力、黏附能力显著受损(分别为53±8vs42±6,0.49±0.12vs0.37±0.02,均P<0.05),细胞上清液SOD水平显著下降(21.95±1.43vs14.76±3.99,P<0.01),MDA水平显著升高(3.72±0.30vs5.57±0.64,P<0.01);黄芪甲苷干预24 h后,显著改善了EPCs的增殖能力、黏附能力,且黄芪甲苷各组SOD水平显著提高,MDA水平显著降低。结论黄芪甲苷对Ox-LDL损伤后EPCs有显著保护作用,其机制可能与抗氧化损伤有关。Objective To observe the effect of astragaloside on oxidative low-density lipoprotein（Ox-LDL） mediated oxidative damage of endothelial progenitor cells（EPCs）.Methods Peripheral blood mononuclear cells（PBMCs） were isolated by Ficoll density gradient centrifugation,and EPCs were identified by flow cytometry.Adherent cells were collected after seven-day incubation and randomly divided into the normal control group,the Ox-LDL group（as the model group,at the dose of 100 μg/mL）,the low,middle,and high astragaloside groups（with 100 μg/mL Ox-LDL plus 2,10,and 50 μg/mL astragaloside）.Twenty-four h later,the proliferation and adhesion capabilities of EPCs were observed using MTT colorimetry and the adhesion capability detection.Levels of superoxide dismutase（SOD） and malonaldehyde（MDA） in the cell supernate of each group were determined.Results After Ox-LDL damage,the proliferative and adhesive capacities of EPCs were significantly injured（53±8 vs 42±6,0.49±0.12 vs 0.37±0.02,both P0.05）.The SOD content obviously decreased（21.95±1.43 vs 14.76±3.99,P0.01）,the MDA content obviously increased（3.72±0.30 vs 5.57±0.64,P0.01）.After intervened by astragaloside for 24 h,the proliferative and adhesive capacities of EPCs were significantly improved.The SOD contents of astragaloside intervention groups were obviously improved and the MDA content obviously lowered.Conclusions Astragaloside showed significant protection on Ox-LDL damaged EPCs.Its mechanism might be correlated with antioxidative damage.

关 键 词：黄芪甲苷 内皮祖细胞 氧化低密度脂蛋白 超氧化物歧化酶 丙二醛 

分 类 号：R285.5[医药卫生—中药学]