长期给予睾酮致大鼠生精小管伴有生精细胞排列疏松  

Testosterone-induced spermatogenic impairment is associated with looser arrangement of spermatogenic cells in rats

作　　者：邓显忠[1] 廖波[1] 孔令淑[2] 杨正伟[2] 

机构地区：[1]川北医学院附属医院泌尿外科,四川南充637000 [2]川北医学院形态定量研究室,四川南充637000

出　　处：《中华男科学杂志》2011年第6期507-510,共4页National Journal of Andrology

基　　金：四川省卫生厅科研项目(040019);四川省青年科技基金(04ZQ026-025)~~

摘　　要：目的:确定睾酮致大鼠睾丸内睾酮抑制因而精子发生障碍是否伴有生精细胞排列疏松。方法:成年雄性SD大鼠肌注十一酸睾酮[19 mg/(kg.15 d)]130 d后取睾丸组织块,作甲基丙烯酸树脂包埋切片,观察睾丸的组织学变化。结果:除精子形成、精子释放障碍等改变以外,11.5%的生精小管轮廓内生精细胞的排列明显较疏松,成串、成束或成团的生精细胞(主要是精母细胞和精子细胞)之间出现朝向小管腔走行的放射状裂隙。结论:生精细胞排列疏松是大鼠睾丸内睾酮抑制所致重要组织学改变之一。Objective： To determine whether testosterone-induced intra-testicular testosterone withdrawal and therefore spermatogenic impairment is associated with looser arrangement of spermatogenic cells in rats.Methods： Adult male SD rats received intramuscular injection of testosterone undecanoate at 19 mg/（kg·15 d） for 130 days,and then testicular tissue blocks were obtained for the preparation of methacrylate resin-embedded sections and observation of the changes in testicular histology.Results： Apart from such changes as impaired spermiogenesis and spermiation,apparently looser arrangement of spermatogenic cells was seen in 11.5% of the seminiferous tubule profiles,with radial cracks（empty spaces） running towards the tubule lumen being formed between lines,bundles or groups of spermatogenic cells（mainly spermatids and spermatocytes）.Conclusion： Looser arrangement of spermatogenic cells is one of the key histological changes resulting from intra-testicular testosterone withdrawal in rats.

关 键 词：十一酸睾酮 睾丸 生精小管 生精细胞 大鼠 

分 类 号：R321.1[医药卫生—人体解剖和组织胚胎学] R332[医药卫生—基础医学]