Annexin A7在精原干细胞中的表达研究  

Expression of Annexin A7 in spermatogonial stem cells

作　　者：于春梅[1] 张平[1] 王静[1] 刘明兮[1] 王晖[1] 周作民[1] 沙家豪[1] 

机构地区：[1]南京医科大学江苏省生殖医学重点实验室,江苏南京210029

出　　处：《中华男科学杂志》2011年第6期516-522,共7页National Journal of Andrology

摘　　要：目的:探讨Annexin A7在睾丸中的表达,尤其是在精原细胞各亚型中的分布。方法:采用原核表达法制备了Annexin A7的重组蛋白,质谱鉴定后用其吸附Annexin A7抗体,并用W estern印迹及免疫组化的方法检测了Annexin A7的表达。结果:研究发现Annexin A7于青春期前的小鼠睾丸中主要在精原细胞中表达,并有一定发育依赖性;在成年睾丸中主要表达于As及Apr精原细胞,且与分化型精原干细胞(SSCs)的分子标志Stra8的共表达也进一步证实上述结果。结论:Annexin A7是As及Apr精原干细胞的内在因子,可参与SSCs的生物学功能。Objective： To study the expression of Annexin A7 in the mouse testis,especially in different types of spermatogonia.Methods： We prepared Annexin A7 recombinant protein using prokaryotic expression,adsorped the Annexin A7 antibody with it after identified by mass spectrometry,and detected the expression of Annexin A7 by Western-blot and immunohistochemistry.Results： Annexin A7 was expressed in a development-dependent manner in the spermotogonia of the prepubertal mice and in the type-A single（As） and type-A paired（Apr） spermatogonia of adult mice.These results were confirmed by the co-localization of Annexin A7 and Stra8,a known determinant of differentiated spermatogonial stem cells（SSCs）.Conclusion： Annexin A7 is the internal factor of As and Apr spermatogonia,which might be involved in the biological functions of SSCs.

关 键 词：AnnexinA7 精原干细胞(SSCs) As精原细胞 Apr精原细胞 

分 类 号：R321.1[医药卫生—人体解剖和组织胚胎学]