Murine Cytomegalovirus IE3 Protein Interacts with Ankrd17  

作　　者：王慧 刘兴楼 舒赛男 张菊 黄永建 方峰 

机构地区：[1]Department of Pediatrics,Tongji Hospital,Tongji Medical College,Huazhong University of Science and Technology

出　　处：《Journal of Huazhong University of Science and Technology(Medical Sciences)》2011年第3期285-289,共5页华中科技大学学报（医学英德文版）

基　　金：supported by a grant from National Natural Sciences Foundation of China (No. 30671859)

摘　　要：Murine cytomegalovirus（MCMV） IE3 protein is a multifunctional viral protein that inter-acts with several target proteins of both viral and host cellular origin.To investigate the biological func-tion of IE3 in the pathogenesis of the brain disorders caused by CMV,a screening for host cellular pro-teins that could interact with IE3 was performed.By yeast two-hybrid screening,ankyrin repeats do-main 17（Ankrd17,also known as Gtar） was identified as a host factor that could interact with IE3.This interaction was verified by yeast two-hybrid assay and chemiluminescent co-immunoprecipitaion.Map-ping analysis suggested that the 1-148 residues of IE3 were responsible for the interaction.These results suggested that the interaction between Ankrd17 and IE3 may play a key role in the pathogenesis of MCMV-associated disease.Murine cytomegalovirus（MCMV） IE3 protein is a multifunctional viral protein that inter-acts with several target proteins of both viral and host cellular origin.To investigate the biological func-tion of IE3 in the pathogenesis of the brain disorders caused by CMV,a screening for host cellular pro-teins that could interact with IE3 was performed.By yeast two-hybrid screening,ankyrin repeats do-main 17（Ankrd17,also known as Gtar） was identified as a host factor that could interact with IE3.This interaction was verified by yeast two-hybrid assay and chemiluminescent co-immunoprecipitaion.Map-ping analysis suggested that the 1-148 residues of IE3 were responsible for the interaction.These results suggested that the interaction between Ankrd17 and IE3 may play a key role in the pathogenesis of MCMV-associated disease.

关 键 词：CYTOMEGALOVIRUS IE3 protein yeast two-hybrid system ankyrin repeats domain 17 

分 类 号：R373[医药卫生—病原生物学]