中国北方汉族人群IL-18基因-607C/A和-137G/C多态与心肌梗死的关联性  

Association of interleukin-18 gene promoter polymorphisms with myocardial infarction in a Han population of northern China

作　　者：张效林[1] 裴芳[1] 黄明方[1] 闫承慧[1] 康建[1] 梁振洋[1] 韩雅玲[1] 

机构地区：[1]沈阳军区总医院心内科,辽宁沈阳110016

出　　处：《心脏杂志》2011年第3期331-335,共5页Chinese Heart Journal

基　　金：军队"十一五"计划科技攻关课题资助项目(06G021)

摘　　要：目的:近年研究发现白细胞介素-18(Interleukin18,IL-18)在冠心病(CAD)的发生、发展及粥样斑块破裂的过程中起重要作用。本研究探讨IL-18基因-607C/A和-137G/C单核苷酸多态与中国北方汉族人群心肌梗死(MI)的关系。方法:采用序列特异性引物聚合酶链反应对432例对照组和468例MI患者进行检测,分析IL-18基因-607C/A和-137G/C单核苷酸多态的基因型和等位基因分布情况。结果:IL-18基因-607C/A单核苷酸多态3种基因型(CC型,CA型和AA型)在对照组分布频率分别为20.8%,50.9%和28.2%,在MI组分别为36.3%,44.9%和18.8%,IL-18基因-607C/A多态和中国北方汉族人群MI的发生显著相关(P<0.05)。IL-18基因-137G/C单核苷酸多态三种基因型(GG型,GC型和CC型)在对照组分布频率分别为71.3%,26.8%和1.9%,在MI组分别为75.2%,23.9%和0.9%,IL-18基因-137G/C单核苷酸多态与中国北方汉族人群MI无相关性(P=0.133)。两组间的基因型分布皆符合Hardy-Weinberg平衡定律。Logistic回归校正性别、年龄、体质量指数、吸烟、高血压病、高脂血症、糖尿病等CAD易患因素后,IL-18基因-607C/A多态仍是MI发病的独立的危险因素(P<0.05)。IL-18基因-607C/A和-137G/C单核苷酸多态组成的CG单体型与和MI的危险性呈正相关,而AC单体型与MI的危险性呈负相关。结论:IL-18基因-607C/A多态与中国北方汉族人群中与MI的发生独立相关。AIM： To investigate the association between IL-18 promoter functional polymorphisms （-607C/A and -137G/C） and myocardial infarction （MI） in a Han population of northern China. METHODS： A case-control study was conducted in 468 patients with MI and 432 controls with normal results of coronary angiograms. Genotyping was performed by sequence-specific primer-polymerase chain reaction. RESULTS： Genotype frequencies of CC, CA and AA of the IL-18 -607C/A polymorphism were, respectively, 20. 83% , 50.93% and 28.24% in the controls, and 36. 32% , 44. 87% and 18.81% in MI patients. Genotype frequencies of GG, GC and CC of the IL-18 -137G/C polymorphism were, respectively, 71.30%, 26. 85% and 1.85% in controls and 75.21%, 23.93% and 0. 86% in MI patients. Significant differences were observed in the genotype and allele distribution of -607C/A polymorphism of the IL-18 gene between cases and controls （P 〈 0.05 ）. Logistic regression analysis with adjustments for other well-established risk factors revealed that the -607C allele carriers had a significantly increased risk of MI compared with the non-carriers （P 〈 0.05 ）. No relationship between -137G/C polymorphism and MI was found in this study （P = 0. 133）. Compared with the AC haplotype, the CG haplotype was associated with reduced oecurrence of MI. CONCLUSIONS： This study shows for the first time that the IL-18 gene promoter -607C/A polymorphism can be considered a genetic risk factor for MI in a Han population of northerb China. CG haplotype is associated with MI occurrence.

关 键 词：冠状动脉疾病 心肌梗死 基因 单核苷酸多态性 

分 类 号：R541.4[医药卫生—心血管疾病]