Slingshot-1L在哮喘急性发作期外周血嗜酸粒细胞中的定位与表达变化(英文)  

Localization and expression of Slingshot-1L in peripheral eosinophils from patients with acute asthma exacerbation

作　　者：张卫珍[1] 赵海金[1] 邹飞[2] 李文军[2] 蔡绍曦[1] 

机构地区：[1]南方医科大学南方医院呼吸科,广东广州510515 [2]南方医科大学公共卫生和热带医学学院,广东广州510515

出　　处：《南方医科大学学报》2011年第6期928-932,共5页Journal of Southern Medical University

基　　金：Supported by National Natural Science Foundation of China (30270593)~~

摘　　要：背景嗜酸粒细胞(EOS)在哮喘气道炎症中起着很重作用,我们前期发现Slingshot-1L(SSH-1L)在急性发作期哮喘病人外周血EOS表达明显增高,可能在EOS的激活、迁移中发挥重要作用。目的 探讨SSH-1L在哮喘急性发作期病人外周血EOS的定位及治疗后的表达及功能变化。方法 招募4例未经糖皮质激素治疗的急性发作期哮喘门诊病人,在治疗前及吸入糖皮质激素3个月后抽取外周静脉血各30 ml进行EOS的分离纯化并计数,应用RT-PCR和Western Blotting方法分别在基因及蛋白质水平检测哮喘外周血EOS SSH-1L的表达变化;应用免疫荧光观察其中1例病人SSH-1L在外周静脉血EOS 中的定位。结果 免疫荧光显示外周血 EOS 的 SSH-1L 定位在胞浆中,在贴近胞壁处丰富表达。急性发作期哮喘病人糖皮质激素治疗前后外周血EOS RT-PCR扩增SSH-1L基因的SSH-1L/β-Actin灰度值比:治疗前0.7403±0.1124,治疗后0.4101±0.0363,治疗前后有显著差异(P=0.001);急性发作期哮喘病人吸入糖皮质激素治疗前后外周血EOS蛋白的SSH-1L/β-actin 灰度值比分别为 0.3410±0.1337 和 0.1543±0.0551,治疗后明显降低(P=0.039)。结论 SSH-1L 可能在急性发作期哮喘病人外周血EOS的激活、迁移上发挥作用,糖皮质激素可能通过抑制哮喘急性发作期EOS的SSH表达而抑制EOS的激活、迁移起到治疗作用。Background Eosinophils play a pivotal role in asthmatic airway inflammation. We previously found a significantly high expression of Slingshot-1L （SSH-1L） in peripheral eosinophils in acute exacerbations of asthma. Objective To investigate the expression and localization patterns of SSH-1L in peripheral blood eosinophils of asthmatic patients and their changes after treatment with inhaled corticosteroids. Methods We recruited 4 outpatients with acute exacerbations of asthma who received no previous corticosteroid treatment and 1 healthy volunteer. From all the subjects 30 ml peripheral venous blood samples were collected before and after a 3-month treatment with inhaled fluticasone. The eosinophils were isolated, purified and counted, and the expressions of SSH-1L in the eosinophils were examined by RT-PCR and Western blotting. The localization of SSH-1L phosphatases in the peripheral eosinophils was detected by immunofluorescence assay in one patient. Results SSH-1L phosphatases distributed diffusely in the cytoplasm, especially dense near the membrane of the peripheral eosinophils. Glucocorticoids treatment resulted in a significant reduction in both the SSH-1L mRNA expression （0.7403±0.1124 vs 0.4101±0.0363, P=0.001） and SSH-1L protein expression （0.3410±0.1337 vs 0.1543±0.0551, P=0.039）. Conclusions A high expression of SSH-1L in peripheral eosinophils in acute exacerbations of asthma may play a role in the activation and migration of eosinophils. The efficacy of inhaled corticosteroids in asthma control might be partly attributed to a down-regulated expression of SSH-1L

关 键 词：Slingshot-1L 支气管哮喘 嗜酸粒细胞 糖皮质激素 

分 类 号：R562.25[医药卫生—呼吸系统]