凋亡和坏死细胞输注对脓毒症小鼠存活率的影响  

Impact of transfusion of apoptotic and necrotic thymocytes on the survival of mice with sepsis

作　　者：刘占国 张婕[2] 何懿[3] 常平[1] 顾冬生[4] 罗宇维[3] 孙尔维[3] 

机构地区：[1]南方医科大学珠江医院重症医学科,广东广州510282 [2]四川省卫生学校微生物学教研室,四川成都610100 [3]南方医科大学第三附属医院风湿免疫科,广东广州510360 [4]解放军421医院,广东广州510318

出　　处：《南方医科大学学报》2011年第6期960-964,共5页Journal of Southern Medical University

基　　金：国家自然科学基金(30872312)~~

摘　　要：目的研究凋亡和坏死细胞对脓毒症小鼠存活率的影响。方法将BALB/c小鼠分为3组,分别为PBS对照组、凋亡细胞输注组和坏死细胞输注组,输注3d后行盲肠结扎穿孔,制作脓毒症模型,观察脓毒症小鼠的存活率和器官病理损伤。结果 PBS对照组小鼠2周存活率为44.6%,肺脏和肾脏组织出现一定程度的损伤;凋亡细胞输注组1周存活率为69.6%,较对照组显著提高(P=0.012),肺脏和肾脏组织损伤明显减轻;坏死细胞输注组1周存活率为31.6%,较对照组显著下降(P=0.035),肺脏和肾脏组织损伤明显加重。结论脓毒症造模前3d输注凋亡细胞可以减轻器官损伤,提高脓毒症小鼠的存活率,而输注坏死细胞可以加重器官损伤,降低存活率。Objective To investigate the effect of transfusion of apoptotic and necrotic thymocytes prior to sepsis on the survival rate of mice. Method BALB/c mice are divided into 3 groups and received intravenous injection of PBS （control）, apoptotic thymocytes, or necrotic thymocytes. Three days later, cecal ligation and puncture （CLP） were performed to induce sepsis in these mice, and their survival and organ damage were observed. Result The survival rates of mice in PBS group was 44.6% at the end of first week after CLP, and obvious lung and kidney damages were observed. A significant increase in the survival rate was found in apoptotic cell transfusion group （69.6%, P=0.012）, with also lessened lung and kidney damages. The survival rate of mice in necrotic cell transfusion group was only 31.6% at 2 weeks, significantly lower than that in PBS group （P=0.035）, and the lung and kidney damage was even more obvious. Conclusion Transfusion of apoptotic thymocytes 3 days before induction of sepsis can reduce organ damage and improve the survival rate of mice, while necrotic cell transfusion produces the opposite effect

关 键 词：凋亡 坏死 脓毒症 存活率 

分 类 号：R392[医药卫生—免疫学]