人参皂甙Rg1对海马电损伤大鼠的学习记忆功能和海马神经细胞形态的影响  

Effects of ginsenoside Rg1 on learning and memory function and morphology of hippocampal neurons of rats with electrical hippocampal injuries

作　　者：陈志勇[1] 杜天明[1] 陈善成[1] 

机构地区：[1]暨南大学附属第一医院神经外科,广东广州510630

出　　处：《南方医科大学学报》2011年第6期1039-1042,共4页Journal of Southern Medical University

基　　金：广东省中医药局建设中医药强省科研课题(2060112)

摘　　要：目的研究人参皂甙Rg1对海马电损伤SD大鼠的学习记忆功能和海马神经细胞形态的的保护作用。方法选用成年雌性SD大鼠,分成4组:人参皂甙Rg1治疗组、生理盐水组、假手术组和假手术+人参皂甙Rg1治疗组;通过立体定向仪精确定位大鼠海马,导入电极并通直流电损伤海马,制备海马电损伤的大鼠模型。假手术组和假手术+人参皂甙Rg1治疗组不通电,模型制备完成后,人参皂甙Rg1治疗组和假手术+人参皂甙Rg1治疗组用50mg/(kg·d)人参皂甙Rg1灌胃,生理盐水对照组和假手术组均使用用生理盐水灌胃连续14d。给药结束后,通过Morris水迷宫测试观察人参皂甙Rg1对海马电损伤SD大鼠学习记忆功能的保护作用。然后通过灌注固定大鼠大脑神经细胞,并且石蜡切片,对切片进行神经元HE染色和神经元尼氏染色,来观察各组大鼠海马神经元存活状态,神经元细胞的排列情况和神经元尼氏小体的数量。结果人参皂甙Rg1可明显改善海马电损伤SD大鼠学习记忆功能;假手术组和假手术+人参皂甙Rg1治疗组的存活神经元数目没有明显差别(P>0.05),同时生理盐水组的尼氏小体数目明显低于其他各组。结论人参皂甙Rg1可改善海马电损伤SD大鼠学习记忆功能,人参皂甙Rg1对电损伤神经元的的保护作用可能与其保护保护神经细胞结构和排列的完整性有关。Objective To investigate the effect of ginsenoside Rg1 （G-Rg1） on the morphology of the hippocampal neurons of rats with electrical hippocampal injuries and evaluate its protective effects on the learning and memory function. Methods Forty female SD rats were randomly divided into G-Rg1 group, saline group, sham-operated group and G-Rg1＋Sham operation group. Using the stereotactic apparatus, electrical hippocampal injury was induced, not in the two sham groups, by application of direct electrical current, followed by treatments with intragastric administration of G-Rg1 or saline for 14 consecutive days. The learning and memory function of the rats was assessed with Morris water maze test. The viability and arrangement of the hippocampal neurons and the number of Nissl bodies were observed after the treatments. Results Treatment with G-Rg1 significantly improved the learning and memory function of rats with electrical hippocampal injury. The viability of the hippocampal neurons showed no significant changes in the two sham-operated groups （P〉0.05）, and the number of Nissl bodies was much lower in saline group than in the other groups （P〈0.05）. Conclusion G-Rg1 can improve the learning and memory function of rats with electrical hippocampal injury, the mechanism of which is probably associated with its protective effect on the hippocampal neurons against electrical injury

关 键 词：人参皂甙RG1 海马 电损伤 Morris水迷宫 尼氏染色 

分 类 号：R285.5[医药卫生—中药学]