甲基丙烯酸环氧丙酯致人支气管上皮细胞恶性转化相关DNA修复基因表达改变的研究  

Expressing the alteration of DNA repair genes in malignant transformation of human bronchial epithelial cells induced by glycidyl methacrylate

作　　者：李瑛[1] 王全凯[1,2] 王安娜[1,2] 胡洁[1] 许建宁[1,2] 

机构地区：[1]中国疾病预防控制中心职业卫生与中毒控制所,北京100050 [2]中国疾病预防控制中心化学污染与健康安全重点实验室,北京100050

出　　处：《癌变．畸变．突变》2011年第3期161-165,共5页Carcinogenesis,Teratogenesis & Mutagenesis

基　　金：国家自然科学基金资助项目(81072318)

摘　　要：目的:检测甲基丙烯酸环氧丙酯(glycidyl methacrylate,GMA)致人支气管上皮细胞(16HBE)恶性转化相关DNA修复基因表达改变的情况,以探讨GMA致细胞癌变的机制。方法:采用"基因组稳定和DNA修复基因芯片"分析检测GMA诱导16HBE细胞恶性转化过程中,经鉴定具备恶性细胞表型特征的第30代转化细胞DNA修复基因表达的改变。结果:GMA转化第30代细胞与同代龄对照细胞比较,在113个基因中有8个基因的表达有显著差异,其中7个基因表达上调(ratio>2),分别是NBN、RAD50、RAD51、OGG1、XAB2、TOP3A、TNKS;NEIL2基因表达下调(0<ratio<0.5)。结论:GMA致16HBE细胞恶性转化是一个多基因参与涉及多通路的复杂过程,DNA修复基因表达的改变可能是该过程中重要的分子事件。OBJECTIVE：To observe the expression change of malignant transformation-related DNA repair genes in human bronchial epithelial cells（16HBE）induced by glycidyl methacryiate（GMA）.METHODS：The expression of DNA repair genes in 30^（th）generation cells which had malignant cell phenotype was evaluated with the gene chip of＂human genome stability/DNA repair microarray＂.RESULTS：Compared with control cells in the 30^（th）generation, eight genes screened from 113 genes showed different expressions in cells induced by GMA.Among the eight genes,there were 7 genes（NBN,RAD50,RAD51,OGG1,XAB2,TOP3A,TNKS）with increased expression（ratio2）and 1 gene （NEIL2）with lower expression（0ratio0.5）.CONCLUSION：Malignant transformation in human bronchial epithelial cells（16HBE）induced by GMA involved multi-genes and multi-pathways,so DNA repair genes changes may be the important molecular event in the process.

关 键 词：甲基丙烯酸环氧丙酯 人支气管上皮细胞 细胞恶性转化 DNA修复基因 基因表达 

分 类 号：R34[医药卫生—基础医学] Q754[生物学—分子生物学]