真菌提取物AMH的肿瘤化学预防活性组分追踪  

Identification of the cancer chemoprevention component in fungus plant extract AMH

作　　者：贺小琼[1] 李凝诗[1] 李凤[1] 赵燕[1] 武金聪[1] 朱启应[1] 原茵[1] 

机构地区：[1]昆明医学院公共卫生学院,云南昆明650500

出　　处：《癌变．畸变．突变》2011年第3期166-170,共5页Carcinogenesis,Teratogenesis & Mutagenesis

基　　金：国家自然科学基金(30960437);云南省教育厅重点课题基金(09Z0029);云南省科技计划项目(2009FXL001)

摘　　要：目的:对真菌提取物AMH进行活性组分追踪,为分离其活性化学成分、开发抗癌药物提供基础。方法:采用苯并(a)芘诱发小鼠肿瘤实验,评价AMH的5个组分对苯并(a)芘诱发肿瘤的预防作用。结果:苯并(a)芘能诱导昆明种小鼠多个脏器发生肿瘤。AMH 5个组分对苯并(a)芘诱发小鼠肿瘤的作用存在明显差异,与阴性对照组相比,组分A、B、C、E具有促进肺腺瘤发生的作用(P<0.05);组分B和E能抑制苯并(a)芘诱导的脏器肿瘤(P<0.05);组分D具有抑制苯并(a)芘诱发小鼠脏器肿瘤和肺腺瘤的作用(P<0.01),是AMH中具有肿瘤化学预防作用的活性组分。结论:AMH组分D具有肿瘤化学预防作用,是下一步活性追踪的功效组分。OBJECTIVE：To identify the cancer chemoprevention component in fungus plant extract AMH, which has been proved having antimutagenic and cancer chemopreventive effect.Present study may provide scientific data for the further separation of bioactive agents and for anticancer drug development.METHODS：The benzo（a）pyrene-induced oncogenesis assay was used to evaluate the cancer chemopreventive effect of the 5 components separated from fungus extract AMH.RESULTS：Benzo（a）pyrene could induce organ cancers and lung adenoma in Kunming mice. Compared with the control group,components A,B,C and E could promote the occurrence of lung adenoma（P0.05）. Components B and E could inhibit the occurrence of cancers induced by benzo（a）pyrene（P0.05）whilst component D could inhibit both cancers and lung adenoma induced by benzo（a）pyrene（P0.01）.Component D was the bioactive component which had cancer chemopreventive effect.CONCLUSION：Component D had cancer chemopreventive effect. It was the functional component worthy of further identification of the bioactive agents.

关 键 词：真菌提取物AMH 肿瘤 苯并(A)芘 肺腺瘤 

分 类 号：R34[医药卫生—基础医学]