维生素A和E抗辐射氧化损伤作用的研究  

The effect of vitamin A and vitamin E against Co^(60) radiationinduced oxidative injury

作　　者：王欣[1] 王璐[1] 陈宏莉[1] 柏桦[1] 王钊[1] 刘江正[1] 海春旭[1] 

机构地区：[1]第四军医大学军事毒理学教研室,陕西西安710032

出　　处：《癌变．畸变．突变》2011年第3期181-185,共5页Carcinogenesis,Teratogenesis & Mutagenesis

摘　　要：目的:探讨维生素A(vitamin A,V_A)和维生素E(vitamin E,V_E)抗辐射氧化损伤的相互作用。方法:实验分为V_A、V_E抗辐射氧化损伤实验和V_A、V_E联合应用抗辐射氧化损伤实验。前者设阴性对照组、阳性对照组、V_A(30mg/kg)组、V_E(30mg/kg)组及V_A+V_E半量(均为15mg/kg)联合用药组。各组大鼠每天用受试物灌胃1次,连续灌胃6d,第7天除阴性对照组外其它各组大鼠均以6Gy的^(60)Co照射,再继续灌胃3d后处死大鼠,取血、脑和肝组织,测定MDA、SOD、CAT等指标。V_A、V_E联合应用抗辐射氧化损伤实验设阴性对照组、阳性对照组、V_E组(30mg/kg)、V_E(30mg/kg)与4个不同V_A剂量(分别为2.5、5、10、20mg/kg)的联合应用组,共7组。各组大鼠的处理同前述。结果:与阳性对照相比,单独补充V_A、V_E均可以对辐射损伤产生一定的保护作用,血清、脑、肝脏的MDA含量均显著降低(P均<0.05),各种抗氧化酶活性均显著升高(P均<0.05)。与阴性对照组比较,固定V_E剂量(30mg/kg)与不同V_A剂量联合应用时,大鼠血清、脑、肝脏的MDA含量均显著增加,CAT和SOD活性降低,脑、肝脏组织中V_E含量显著减少(P均<0.05),并具有一定的剂量-效应关系。V_A、V_E联合应用时产生的抗氧化作用明显弱于V_A、V_E单独使用的效果。结论:维生素A和维生素E均具有抗辐射氧化损伤的作用,但在一定的剂量下,V_A、V_E联合应用可以产生拮抗作用。OBJECTIVE：To study the antagonistic effects of vitamin A and vitamin E against Co^（60）radiationinduced oxidative injury.METHOD：Experiment 1：Rats were divided into five groups,including control, radiation,radiation＋vitamin A,radiation＋vitamin E and radiation ＋ half dose of vitamin A and vitamin E.Before exposure to radiation,rats were treated with intragastrically with 30 mg/kg vitamin A,30 mg/kg vitamin E,or 15 mg/kg vitamin A＋15 mg/kg vitamin E.Experiment 2：Rats were divided into seven groups,including control, radiation,radiation＋30 mg/kg vitamin E,radiation＋30 mg/kg vitamin E＋different concentrations of vitamin A,ranging from 2.5 mg/kg to 20 mg/kg.Before the exposure to radiation,rats received intragastrically 30 mg/kg vitamin E,or 30 mg/kg vitamin E＋different concentration of vitamin A.At day 7,rats were exposed to 6 Gy Co^（60）radiation.At day 10,the rats were sacrificed,and serum and tissues were collected for analysis.RESULTS：Vitamin A and vitamin E alone could protect the rats against the injury induced by radiation.When the concentration of vitamin E was fixed at 30 mg/kg,different concentrations of vitamin A resulted in the increase of MDA,the decrease of SOD and CAT activities and the content of vitamin E,in a dose-dependent manner.CONCLUSION：Under certain concentration,vitamin A and vitamin E could produce antagonistic effect.

关 键 词：维生素A 维生素E 辐射损伤 丙二醛 超氧化物歧化酶 过氧化氢酶 

分 类 号：R818.74[医药卫生—放射医学]