一次性筷子、牙签及纸杯浸提液对小鼠L-929细胞的毒性作用  

Toxic effects of the extracted solution of disposable chopstick toothpick or cup in murine L-929 cells

作　　者：陈思凡[1,2] 李文学[1] 贾慧影[2] 陈建玲[1,2] 杨光宇[1] 朱伟[1,2] 

机构地区：[1]广州市疾病预防控制中心,广东广州510080 [2]中山大学公共卫生学院,广东广州510080

出　　处：《癌变．畸变．突变》2011年第3期209-212,共4页Carcinogenesis,Teratogenesis & Mutagenesis

基　　金：广州市科技计划项目(2009Z1-E061)

摘　　要：目的:评价一次性筷子、牙签及纸杯浸提液对小鼠L-929细胞的毒性作用。方法:参照国家标准,制备一次性筷子、牙签及纸杯等样品浸提液,采用样品浸提液配制培养液培养小鼠L-929细胞,在光镜下观察细胞生长形态,采用细胞计数法计算细胞失贴壁率,采用WST-1法测定细胞活性并计算细胞增殖度。结果:与对照组相比,采用浸提液配制的细胞培养液培养细胞4d或7d后,各组细胞出现凋亡或坏死征象,细胞失贴壁率明显升高(P<0.05),细胞活性及细胞增殖度明显下降(P<0.05)。结论:一次性筷子、牙签及纸杯用具等能影响L-929细胞生长及增殖,可能具有潜在的毒性作用。OBJECTIVE：To evaluate the toxic effects of the extracted solution of disposable chopstick, toothpick or cup in murine L-929 cells.METHODS：According to the national standards,we prepared cell culture medium with the extracted solution of disposable chopsticks,toothpicks or cups.The cell morphology of murine L-929 cells was examined microscopically and cell viability was detected with WST-1 staining method.The detached cell ratios and the relative growth rate of L-929 cells were calculated.RESULTS：Compared with the control,after treatment for 4 days or 7 days,cells in the experimental groups showed signs of apoptosis or necrosis.The detached cell ratios were increased significantly（P0.05）,while cell viability and relative growth rate declined significantly（P0.05）. CONCLUSION：Treatments with extracted solution of disposable chopstick,toothpick or cup could affect cell growth and proliferation,thus probably possess potential toxic effects.

关 键 词：牙签 一次性筷子 一次性纸杯 细胞毒性 

分 类 号：R994.6[医药卫生—毒理学]