Pregnant phenotype in aquaporin 8-deficient mice  

作　　者：Xiao-yan SHA Zheng-fang XIONG Hui-shu LIU Zheng ZHENG Tong-hui MA 

机构地区：[1]Dept of Obstetrics, Guangzhou Women and Children's Medical Centre, Guangzhou Medical College, Guangzhou 510623, China [2]Central Research Laboratory, Jilin University Bethune Second Hospital, Changchun 130024, China

出　　处：《Acta Pharmacologica Sinica》2011年第6期840-844,共5页中国药理学报（英文版）

摘　　要：Aim- Aquaporin 8 （AQP8） is expressed within the female reproductive system but its physiological function reminds to be elucidated. This study investigates the role of AQP8 during pregnancy using AQP8-knockout （AQP8-KO） mice. Methods.＂ Homozygous AQP8-KO mice were mated, and the conception rate was recorded. AQP8-KO pregnant mice or their offspring were divided into 5 subgroups according to fetal gestational day （7, 13, 16, 18 GD） and newborn. Wild type C57 pregnant mice served as the control group. The number of pregnant mice, total embryos and atrophic embryos, as well as fetal weight, placental weight and placental area were recorded for each subgroup. The amount of amniotic fluid in each sac at 13, 16, and 18 GD was calculated. Statistical significance was determined by analysis of variance of factorial design and chi-square tests. Results： Conception rates did not differ significantly between AQP8-KO and wild type mice. AQP8-KO pregnant mice had a significantly higher number of embryos compared to wild type controls. Fetal/neonatal weight was also significantly greater in the AQP8-KO group compared to age-matched wild type controls. The amount of amniotic fluid was greater in AQP8-KO pregnant mice than wild type controls, although the FM/AFA （fetal weight/amniotic fluid amount） did not differ. While AQP8-KO placental weight was significantly larger than wild type controls, there was no evidence of placental pathology in either group. Conclusion： The results suggest that AQP8 deficiency plays an important role in pregnancy outcome.Aim- Aquaporin 8 （AQP8） is expressed within the female reproductive system but its physiological function reminds to be elucidated. This study investigates the role of AQP8 during pregnancy using AQP8-knockout （AQP8-KO） mice. Methods.＂ Homozygous AQP8-KO mice were mated, and the conception rate was recorded. AQP8-KO pregnant mice or their offspring were divided into 5 subgroups according to fetal gestational day （7, 13, 16, 18 GD） and newborn. Wild type C57 pregnant mice served as the control group. The number of pregnant mice, total embryos and atrophic embryos, as well as fetal weight, placental weight and placental area were recorded for each subgroup. The amount of amniotic fluid in each sac at 13, 16, and 18 GD was calculated. Statistical significance was determined by analysis of variance of factorial design and chi-square tests. Results： Conception rates did not differ significantly between AQP8-KO and wild type mice. AQP8-KO pregnant mice had a significantly higher number of embryos compared to wild type controls. Fetal/neonatal weight was also significantly greater in the AQP8-KO group compared to age-matched wild type controls. The amount of amniotic fluid was greater in AQP8-KO pregnant mice than wild type controls, although the FM/AFA （fetal weight/amniotic fluid amount） did not differ. While AQP8-KO placental weight was significantly larger than wild type controls, there was no evidence of placental pathology in either group. Conclusion： The results suggest that AQP8 deficiency plays an important role in pregnancy outcome.

关 键 词：AQP8 gene knockout mice pregnancy outcome female reproductive system 

分 类 号：S823.36[农业科学—畜牧学] Q51[农业科学—畜牧兽医]