快速起搏对家兔心房肌钙通道表达的影响及螺内酯干预的研究  

Influence of Rapid Atrial Pacing on the Expression of α_1c and β_1 Subunit of L-type Calcium Channel and the Protective Effect of Spironolactone

作　　者：王联发[1] 顾磊[1] 吴振西[1] 罗骏[2] 张淑华[2] 

机构地区：[1]中国人民解放军105医院心内二科,合肥230001 [2]江西省人民医院心血管研究所,南昌330006

出　　处：《医学综述》2011年第12期1873-1875,共3页Medical Recapitulate

摘　　要：目的观察螺内酯对快速心房起搏对家兔心房肌细胞L型钙离子通道亚基表达的影响。方法家兔18只随机分为3组,对照组、起搏组及螺内酯组各6只。起搏组和螺内酯组经颈内静脉将电极植入右心房,以600次/min行快速心房起搏8 h,起搏后应用半定量反转录-聚合酶链反应检测心房肌钙离子通道α1C亚基及β1亚基mRNA的表达。结果与对照组比较,起搏组钙离子通道α1C亚基及β1亚基mRNA表达明显减少,分别下降22%和26%,差异有统计学意义(P<0.01);螺内酯组钙离子通道α1C亚基及β1亚基mRNA表达降低明显减少,分别下降13%和17%,与起搏组相比,有统计学意义(P<0.05)。结论螺内酯可预防快速起搏后钙离子通道表达亚基的降低。Objective To investigate the effects of spironolactone on mRNA expression of the L-type calcium channel subunit of the atrium induced by rapid atrial pacing in rabbits.Methods 18 rabbits were randomly divided into 3 groups： control group（n=6）,rapid pacing group（n=6）and spironolactone group（n=6）,electrode catheters were positioned in the right atrium of rapid pacing group and spironolactone group.High-frequency atrial pacing of 600 bpm was started and maintained for 8 hours.Right atrium tissue was obtained for measurement of mRNA expression of L-typecalcium channel subunits by reverse transcription-polymerase chain reaction（RT-PCR）.Results Compared with the control group,the mRNA of α1C and β1 of the rapid pacing group decreased 22% and 26%（P0.01）;the mRNA of α1C and β1 of the spironolactone group only decreased 13% and 17%,compared with the rapid pacing group,there were significant differences（P0.05）.Conclusion The spironolactone can attenuate the down-regulation of L-type calcium channel of atrium induced by the rapid atrial pacing.

关 键 词：心房颤动 螺内酯 快速心房起搏 L型钙离子通道 

分 类 号：R541.7[医药卫生—心血管疾病]