肌间隙移植明胶海绵获取成体干细胞  

Adult Stem Cells Harvested by Implanting Gelatin Sponges into Spatium Intermusculare

作　　者：武晓云[1] 王世立[1] 牟忠祥 

机构地区：[1]卫生部生物技术药物重点实验室山东省医药生物技术研究中心,济南250062 [2]山东省中医药大学附属医院,济南250011

出　　处：《中国生物工程杂志》2011年第6期6-11,共6页China Biotechnology

基　　金：国家自然科学基金(30940065;30571766);山东省自然科学基金(Y2008C06)资助项目

摘　　要：通过移植生物材料到小鼠体内而建立一种新的干细胞获取方法。移植明胶海绵到小鼠后肢的大腿内侧肌间隙,12天后分离明胶海绵中的迁移细胞。海绵来源干细胞形态、增殖能力、多向分化能力与骨髓源干细胞相似,但海绵中成纤维细胞集落形成单位的比例(82.2±10.6/105)远远高于骨髓中的比例(43.7±7.4/106)(P<0.05)。此外,骨髓移植实验证实了迁移细胞中的干细胞来源于外周循环血,而且这种方法可以实现自体细胞治疗,因此有潜力应用于临床。A new method to harvest stem cells was established by implanting biomaterials into mice. Gelatin Sponges （GS） were implanted into the spatium intermusculare of mice hind limbs. A large number of migrating cells were isolated from the transplanted biomaterials at 12 days after implanting. The characteristics of adherent ceils were similar to that of bone marrow （BM） stem cells, including morphology, proliferation potential and muhilineage differentiation capacity. The frequency of CFU-F in GS （82.2 ± 10. 6/10^5） was much higher than in BM （43.7 ± 7.4/10^6 ） （ P 〈 0. 05 ）. In additional, BM transplantation demonstrated that stem cells in the GS originated from the PB. Moreover, it is possible for autologous cell therapy, so this method may be a promising alternative for the clinical application.

关 键 词：明胶海绵 成体干细胞 细胞培养 分化 

分 类 号：Q819[生物学—生物工程]