检索规则说明:AND代表“并且”;OR代表“或者”;NOT代表“不包含”;(注意必须大写,运算符两边需空一格)
检 索 范 例 :范例一: (K=图书馆学 OR K=情报学) AND A=范并思 范例二:J=计算机应用与软件 AND (U=C++ OR U=Basic) NOT M=Visual
作 者:邓省亮[1,2] 李平[1] 刘洪斌[1] 程玛丽[3] 于洪侠[1] 陈刚[1] 杨曙明[1]
机构地区:[1]中国农业科学院农业质量标准与检测技术研究所,北京100081 [2]江西省科学院微生物研究所,南昌330029 [3]北京农学院动物科学技术学院,北京102206
出 处:《中国生物工程杂志》2011年第6期106-110,共5页China Biotechnology
基 金:国家"863"计划(2007AA10Z438);科技部科技支撑专项(2009BADB7B07)资助项目
摘 要:利用4-溴丁酸乙酯对小分子半抗原己烯雌酚(DES)进行活化,引入羧基活性基团,应用活泼酯法将其与牛血清白蛋白(BSA)偶联,合成DES-CP-BSA完全抗原,免疫新西兰长耳白兔,制备特异性抗体。结果显示:成功制备了DES完全抗原,且由此获得了特异性的DES抗体,效价达1.28×105,与己烷雌酚、双烯雌酚的交叉反应分别为9.3%和1.1%,与雌二醇无交叉反应。在此基础上建立了间接竞争ELISA检测方法,IC50为7.0 ng/ml,检测限达0.3 ng/ml,可以满足动物性食品中DES残留检测的需要。Diethylstilbestrol was activated by ethyl 4-bromobutyrate to introduce carboxyl group and then conjugated to BSA via EDC/NHS as immunogen, New Zealand rabbits were immunized and antisera were prepared. The results showed that complete antigen was successfully synthesized and specific antibody was generated, with titer of 1.28 x 105 the antisera cross-reactivity with hexestrol (9.34%), dienestrol ( 1.1% ) and no cross-reactivity with 17[3-estradiol. An indirect competitive enzyme-linked immunosorbent assay (IC-ELISA) was developed for determination of DES. In the linear range between 1 and 30 ng/ml, IC50 of 7 ng/ml and a detection limit of 0. 3 ng/ml for DES were reached. Therefore the assay as screening method can meet the demand of DES residue detection for animal food products.
正在载入数据...
正在载入数据...
正在载入数据...
正在载入数据...
正在载入数据...
正在载入数据...
正在载入数据...
正在链接到云南高校图书馆文献保障联盟下载...
云南高校图书馆联盟文献共享服务平台 版权所有©
您的IP:216.73.216.229