不同频率的机械振动与白细胞介素-1β刺激对兔软骨细胞iNOS及COX-2基因表达的作用  

Effects of mechanical vibration frequency and IL-1β on expressions of catabolism genes in rabbit chondrocytes X

作　　者：肖庭辉[1] 金丹[1] 贺朦 覃承诃[1] 许道荣[1] 罗吉伟[1] 倪国新[1] 余斌[1] 

机构地区：[1]南方医科大学南方医院创作骨科,广州501515

出　　处：《中华创伤骨科杂志》2011年第6期576-578,共3页Chinese Journal of Orthopaedic Trauma

基　　金：国家“973”计划项目子课题题（2009CB930003）

摘　　要：目的研究不同频率的机械振动与白细胞介素-1β（IL-1β）刺激对兔软骨细胞分解代基因表达的作用。方法体外培养兔软骨细胞，应用自制复合振动仪，将振动强度0．5g，不同频率0、45、90Hz的机械振动分别作用于不同类别的软骨细胞，实时PCR（biT—PCR）检测软骨细胞中iNOS、COX-2的含量．结果炎性反应介质IL-1β使软骨绌胞中的iNOS、COX-2基因表达升高，差异有统计学意义（P〈0．01）。在炎性反应介质IL—1β存在的条件下，频率为45、90Hz振动能使软骨细胞中iN—OS、COX-2的表达量下降，差异有统计学意义（P〈0．05）。血九炎性反应介质IL-1β存在的条件下，振动对软骨细胞产生iNOS、COX-2的表达量差异无统计学意义（P〉0．05）。结论炎性反应介质IL-1β能加速兔软骨细胞的玄分化，合遁频率的机械振动能抑制IL-1β诱导的软骨细胞分解代谢基因i—NOS、COX-2的表达。Objective To investigate the effects of mechanical vibration frequency and IL-1 （interleukin-1） on expressions of catabolism genes in eultured rabbit chondrocytes. Methods Chondrocytes were obtained from the joints of a freshly killed 2-month-old rabbit. Six-well plates, half of which con- tained IL-1β, were used to eulture samples. The well plates were vibrated at the frequency and amplitude 0/ 45/90 Hz and 0.5 nm, respectively. After culture and mechanical stimulation, the levels of iNOS and COX-2 in the samples were measured by RT-PCR. Results IL-1β promoted expressions of the iNOS and COX-2 genes in the rabbit chondrocytes （ P 〈 0. 01 ）. In the presence of IL-1β, mechanical vibrations of 45 and 90 ttZ inhibited expression levels of iNOS and COX-2 mRNA, with a significant difterence （P 〈 0.05）. llowever, in the absence of IL-1β, mechanical vibrations of 45 and 90HZ made no significant impact on expressions of iNOS and COX-2 （ P 〉 0. 05）. Conclusion Meehanieal vibration may play an important role in curbing expressions of iNOS and COX-2 genes in the presence of IL-1.

关 键 词：软骨细胞 兔 分解代谢 机械振动 白细胞介素-1Β 

分 类 号：R285.5[医药卫生—中药学]