大鼠创伤性脑损伤后脑组织钙周期素结合蛋白的表达及意义  

Expression and significance of calcyclin binding protein in rat brain tissues after traumatic brain injury

作　　者：梁明[1] 贺晓生[1] 费舟[1] 章翔[1] 顼红雨[1] 霍军丽[1] 陈晓燕[1] 

机构地区：[1]第四军医大学附属西京医院神经外科,西安710032

出　　处：《中华创伤杂志》2011年第6期559-562,共4页Chinese Journal of Trauma

基　　金：国家自然科学基金资助项目（30971173）

摘　　要：目的探讨大鼠创伤性脑损伤（traumatic brain injury，TBI）后脑组织中钙周期素结合蛋白（calcyclin binding protein，CacyBP）的表达规律及意义。方法雄性sD大鼠60只，按随机数字表法分为正常对照组（10只）和TBI组（50只）；采用头颅侧向旋转致伤方法制作TBI模型，并按伤后大鼠处死时间分为6，24，72h、7，14d共5个亚组，每组10只。采用免疫组化方法检测CacyBP在大鼠脑组织中的表达变化规律和分布情况。CacyBP阳性结果判断标准以细胞浆内出现棕黄染色颗粒为阳性，反之为阴性。结果大鼠脑组织中CacyBP主要分布在海马、齿状回及皮层下神经元的胞浆中。与正常对照组的CacyBP高表达比较，TBI后6h，其表达量明显减少，为最低值（P〈0．01），随后表达量逐渐递增，至损伤后14d其表达量恢复高值，与正常对照组比较，差异无统计学意义（P〉0．05）。结论在TBI后，CacyBP出现一个表达低峰，提示在不同机制影响下其可能对脑损伤的发生、发展发挥重要作用。Objective To investigate the expression and significance of calcyclin binding protein （CacyBP） in the brain of rat model of traumatic brain injury （TBI）. Methods Sixty 60 male SD rats were divided randomly into normal control group （n = 10 ） and TBI group （n = 50）. The TBI model was created by using lateral head rotation device and subdivided into 6 h, 24 h, 72 h, 7 d and 14 d group （ 10 rats per group）. The expression and distribution of CacyBP in the rat brain was investigated immunohistochemically. The presence of the brown stained particles was considered as ＂positive＂ and lack of the stained particles as ＂negative＂. Results CacyBP was mainly distributed in the hippocampus, dentate gyrus and cortical neuron cytoplasm. Compared with the high level expression of CacyBP in the normal control group, the expression of CaeyBP was decreased to the lowest in the rat brain at 6 h post TBI （ P 〈 0.01 ） , became stronger gradually at 24 hours and recovered to normal at day 14, with no statistical difference compared with normal control group （P 〉 0.05 ）. Conclusion The lowest bevel expression of CacyBP after TBI indicates that CaeyBP may play an important role in development of brain injury under effect of different mechanisms.

关 键 词：脑损伤 海马 齿状回 钙周期素结合蛋白 

分 类 号：R686[医药卫生—骨科学]