肠致病性大肠杆菌毒力因子EspF对肿瘤坏死因子-α、白细胞介素-6和白细胞介素-8的影响  

Effect of virulence factor EspF from enteropathic E.coli on tumor necrosis factor-α,interleukin-6 and interleukin-8

作　　者：王佳贺[1] 任开明[2] 姜洪芳[1] 白雪[1] 吴宝刚[1] 张萌[1] 何平[1] 

机构地区：[1]中国医科大学附属盛京医院老年病科,辽宁沈阳110004 [2]中国医科大学附属盛京医院胸外科,辽宁沈阳110004

出　　处：《新乡医学院学报》2011年第3期265-267,共3页Journal of Xinxiang Medical University

基　　金：国家自然科学基金资助项目(编号:30770109);辽宁省教育厅资助项目(编号:L2010701)

摘　　要：目的研究肠致病性大肠杆菌(EPEC)毒力因子EspF转染人肠上皮细胞(HIC)株时,血清肿瘤坏死因子-α(TNF-α)、白细胞介素-6(IL-6)和IL-8水平的动态变化及与HIC凋亡的关系。方法以Annexin V FITC/PI双染流式细胞仪检测HIC的凋亡率,并用酶联免疫吸附测定法检测转染0、6、12、24、48 h细胞培养上清液中TNF-α、IL-6和IL-8浓度的变化。结果 EspF转染HIC后TNF-α、IL-6和IL-8活性均显著增加,HIC凋亡率在转染后逐渐增高,HIC凋亡率与TNF-α、IL-6和IL-8的水平呈正相关。结论 EspF可激活多种炎症介质和诱导HIC凋亡,EspF转染HIC后TNF-α、IL-6和IL-8水平的升高在EPEC致病过程中起重要作用。Objective To investigate the relationship between the apoptosis of human intestinal epithelial cells（HIC） and the dynamic changes of tumor necrosis factor-α（TNF-α）,interleukin-6（IL-6） and interleukin-8（IL-8） level when enteropathic E.coli（EPEC） virulence factor carried HIC strain.Methods The apoptosis rate of HIC was detected by Annexin V FITC /PI double dye flow cytometric analysis.The content changes of TNF-α,IL-6 and IL-8 in the supernatant of the cell culture were assessed by enzyme-linked immunosorbent assay（ELISA） at 0,6,12,24 and 48 hours after transfection.Results The activities of TNF-α,IL-6 and IL-8 were increased significantly after EspF transfected HIC,and the apoptosis rate of HIC was increased gradually after transfection.The levels of TNF-α,IL-6 and IL-8 had positive correlation with the apoptosis rate of HIC.Conclusion EspF can activate many inflammatory medium,and induce HIC apoptosis.The level of TNF-α,IL-6 and IL-8 increased after EspF transfected HIC cells which played an important role in the immunopathogenesis of EPEC infection.

关 键 词：肠致病性大肠杆菌 肿瘤坏死因子-Α 白细胞介素-6 白细胞介素-8 人肠上皮细胞 凋亡 

分 类 号：R378.2[医药卫生—病原生物学]