番木瓜果实成熟相关基因的cDNA-AFLP分析及克隆  

cDNA-AFLP Analysis and Cloning of Fruit Ripening-related Genes from Papaya

作　　者：申艳红[1] 陈晓静[1] 卢秉国[1] 何玮毅[1] 

机构地区：[1]福建农林大学园艺学院园艺植物遗传育种研究所,福州350002

出　　处：《园艺学报》2011年第6期1081-1088,共8页Acta Horticulturae Sinica

基　　金：福建省自然科学基金项目(2010J05048)

摘　　要：为了探讨番木瓜果实成熟机理,利用cDNA-AFLP技术对破色期和半黄期的番木瓜果实进行基因差异表达分析,获得了50个与果实成熟相关的差异基因片段,其中28个与GenBank数据库中的功能基因同源,分别参与了果实成熟过程中基因表达调控,DNA与蛋白质合成及运输,蛋白质降解,能量代谢,营养物质代谢和逆境胁迫反应等过程。在差异片段序列基础上设计特异引物,采用cDNA末端快速扩增(RACE)技术成功克隆了4条差异基因cDNA全长,分别命名为CpGMP、CpPAA1、CpPOD和CpMYB。半定量RT-PCR分析结果表明,随着果实的成熟衰老,4个基因的表达量相应变化,说明其可能参与果实的成熟过程。In order to explore molecular mechanism of fruit ripening and the key softening-related genes,cDNA-AFLP technique was used to identify differentially expressed genes during papaya fruit ripening.Fifty transcript-derived fragments（TDFs）were obtained from the analysis of semi-yellow and color-break stage of papaya fruit.Bioinformatics analysis showed that 28 TDFs were homologous to the known functional genes,which could be divided into different functional groups including gene expression regulation,DNA and protein synthesis and transport,protein degradation,energy metabolism,nutrient metabolism and stress responding.Using specific primers designed from the sequences of TDFs,four differential expression genes were cloned by rapid amplification of cDNA ends（RACE）.Semi-quantitative RT-PCR analysis showed that the expression of four genes changed accordingly during fruit ripening,indicating the possible role of these genes in the ripening of papaya.

关 键 词：番木瓜 果实 成熟 CDNA-AFLP 差异表达 RACE 

分 类 号：S667.9[农业科学—果树学] Q78[农业科学—园艺学]