兰州百合鳞茎可溶性酸性转化酶活性检测体系的建立  

Establishment of Detection System for Soluble Acid Invertase Activity in Lilium davidii var.unicolor Bulb

作　　者：孙红梅[1] 何玲[1] 王微微[1] 王春夏[1] 李天来[1] 

机构地区：[1]沈阳农业大学园艺学院设施园艺省部共建教育部重点实验室辽宁省设施园艺重点实验室,沈阳110866

出　　处：《园艺学报》2011年第6期1197-1204,共8页Acta Horticulturae Sinica

基　　金：国家自然科学基金项目(30972023);中国博士后科学基金项目(20090451280);辽宁省重点实验室项目(LS2010148)

摘　　要：为深入研究百合鳞茎蔗糖代谢生理机制,以兰州百合(Lilium davidii var. unicolor)鳞茎外层鳞片为试材,研究了不同种类和pH值的缓冲液、反应时间和反应温度对可溶性酸性转化酶(Soluble acid invertase,SAI,EC3.2.1.26)活性的影响,建立了SAI活性的最佳检测体系。结果表明,最适提取缓冲液为pH8.0的Hepes-NaOH;最佳反应缓冲液为pH4.8的HAc-K3PO4,最适反应温度为40℃,最适反应时间为30min。In order to make a thorough study in sucrose-metabolizing mechanism of lily bulblet,the exterior scales of Lilium davidii var.unicolor bulb were used in present study to explore the effects of buffers with different sorts and pH values,reaction durations and reaction temperatures on SAI activity.Then SAI activity detection system was established.The results indicated that the optimal extraction and reaction buffers were Hepes-NaOH（pH 8.0）and HAc-K3PO4（pH 4.8）,respectively.And SAI has an optimum reaction duration and temperature of 30min and 40℃.

关 键 词：百合 鳞茎 可溶性酸性转化酶 提取条件 反应条件 

分 类 号：S682.2[农业科学—观赏园艺]