一起学校宋内志贺菌群体感染中荧光PCR辅助检测及意义  

The detection of pathogens by fluorescence RCR in a school outbreak of Shigella infection

作　　者：孟建彤[1] 陈恒[1] 苗艳芳[1] 黎明[1] 杨洋[1] 张晓春[1] 李涛[1] 黄薇[1] 

机构地区：[1]成都市疾病预防控制中心微检科,成都610041

出　　处：《中国卫生检验杂志》2011年第6期1424-1425,共2页Chinese Journal of Health Laboratory Technology

基　　金：成都市感染性腹泻病原检测平台的初步构建(成都市CDC青年创新项目;100204)

摘　　要：目的:对一起学校疑似水源性群体感染事件相关标本进行荧光PCR检测,为明确病原、处置疫情提供参考依据。方法:采集现场病例及可疑水源标本,与常规检测同步,以实时荧光PCR试剂盒筛检可疑肠道病毒(诺瓦克病毒)与细菌(产毒素大肠杆菌、沙门菌、痢疾杆菌、副溶血弧菌)。结果:病例标本荧光PCR均检出痢疾杆菌,部分水样增菌4 h检出痢疾杆菌阳性,8 h后则均为阳性。常规检测从病例标本中分得宋内志贺菌。结论:荧光PCR用于暴发疫情实验室辅助检测,准确快速,有利于及早明确病原和及时处理疫情。Objective：To provide the information for pathogens confirmation and epidemic control,detect the samples with real-time fluorescence RCR in a suspected water resource school outbreak.Methods： Collected samples of water and suspicious cases on-site,and screened them of suspected intestinal virus（Norwalk virus） and bacteria（Enterotoxic escherichia coli,Salmonella,Shigella,Vibrio parahaemolyticus） with real-time fluorescence PCR kits when the routine detection was carried out.Results： The cases were positive of Shigella in the patient specimens,some of water specimens incubated for 4h and all the water specimens incubated for 8 h with fluorescence RCR while the Shigella sonnei isolates were obtained from the patients with routine methods.Conclusion： Fluorescent PCR with accuracy and rapidness is applicable for the detection of an outbreak as assistance,which would be conducive to pathogens confirmation and timely control of outbreak.

关 键 词：宋内志贺菌 荧光PCR 辅助检测 

分 类 号：R446.5[医药卫生—诊断学]