紫外可见分光光度法测定不同产地龙牙百合中皂苷含量  

Determination of the Content of Total Saponins in Lilium brownii F E Brown var viridulum Baker.by UV Spectrophotometry

作　　者：傅春燕[1] 刘永辉 李明娟[1] 王晓慧[1] 唐虹[1] 尹文清[3] 

机构地区：[1]邵阳医学高等专科学校,湖南邵阳422000 [2]湖南省新邵县红十字会医院,湖南邵阳422000 [3]广西师范大学化学化工学院药用资源化学与药物分子工程教育部重点实验室,广西桂林541004

出　　处：《广东微量元素科学》2011年第5期55-58,共4页Trace Elements Science

基　　金：湖南省教育厅科研基金(10C0294)

摘　　要：为建立龙牙百合总皂苷的测定方法,并确定不同产地龙牙百合中皂苷的含量,以便控制龙牙百合药材的质量,采用香草醛-高氯酸显色,在535 nm处,利用紫外可见分光光度法测定了10个批次的龙牙百合中总皂苷的含量。结果表明,10个不同产地龙牙百合中皂苷的含量,分别是:8.39%、8.11%、8.24%、7.62%、7.38%、9.02%、8.83%、8.87%、9.21%、9.17%,RSD分别为:1.22%、0.87%、0.93%、1.32%、0.86%、1.37%、0.94%、1.39%、1.55%、1.61%。结果表明,该法准确、可靠、稳定性好,可作为龙牙百合皂苷定量分析的有效方法。To establish a method for the quantitative determination of total saponins in Lilium brownii F E Brown var viridulum Baker by UV spectrophotometry the determination was carried out with chemical reaction, and the total saponins in ten different samples were determined by UV at wavelengths 535 nm. The results showed that the content of the total saponins in ten different samples were 8.39%, 8.11%, 8.24%, 7.62%, 7.38%, 9.02%, 8.83%, 8.87%, 9.21%, 9. 17% respectively, and RSDS of the above ten samples were 1.22%, 0.87%, 0.93%, 1.32%, 0.86%, 1.37%, 0.94%, 1.39%, 1.55%, 1.61%. It concluded the method was simple, accurate and could be used in the quantitative determination of total saponins in Lilium brownii F E Brown var viridulum Baker.

关 键 词：龙牙百合 总皂苷 紫外可见分光光度法 含量测定 

分 类 号：R284.1[医药卫生—中药学] O657.31[医药卫生—中医学]