EV71灭活疫苗(人二倍体细胞)在恒河猴婴猴模型中的免疫保护性分析  

Immunoprotection of Inactivated EV71 Vaccine Against Enterovirus in Neonatal Rhesus Monkey

作　　者：李龙[1] 刘尚允[1] 董承红[1] 赵红玲[1] 王晶晶[1] 张莹[1] 纳锐雄[1] 谢忠平[1] 崔萍芳[1] 王丽春[1] 廖芸[1] 唐东红[1] 高家红[1] 刘龙丁[1] 李琦涵[1] 

机构地区：[1]中国医学科学院&北京协和医学院,医学生物学研究所,云南省重大传染病疫苗研发重点实验室,昆明650118

出　　处：《中国科学：生命科学》2011年第6期439-448,共10页Scientia Sinica(Vitae)

基　　金：国家科技重大专项课题(批准号: 2008ZX10004-014);国家重点基础研究发展计划(批准号: 2011CB504903)资助项目

摘　　要：肠道病毒71型作为引起儿童群体常见传染性手足口病（HFMD）的主要病原，具有导致少量感染个体出现脑炎等神经系统病变以及相关心肺功能衰竭的病理学特性．因此其预防性疫苗的研发具有重要的公共卫生意义．在前期工作的基础上，一种EV71灭活病毒疫苗（人二倍体细胞）在本研究中基于恒河猴婴猴模型进行了相应的免疫保护性分析．以160EU剂量对2～3月龄婴猴进行0，4周免疫后，动物在第4周接受了剂量为10。～CCID50的病毒经呼吸道的攻击．对病毒攻击后动物在14天内的临床症状、血液生物学、器官病原学分布以及病理学检测的动态观察表明，经疫苗免疫的动物未出现对照动物所具有的特征性临床表现，其血液生物学及病理学检测均无异常．同时，器官病原学分布亦呈阴性．结合动物中和抗体的明确增长及对照动物的综合表现分析，本文的工作证实了该EV71灭活病毒疫苗（人二倍体细胞）在恒河猴婴猴体内的免疫保护性．Present study was conducted to evaluate the immunogenicity of one formalin-inactivated EV71 vaccine in neonatal rhesus monkeys （Macaca mulatta）. Groups of four monkeys were immunized with either EV71 vaccine or mock by intra-muscular （IM, using needle） routes. Following primary immunization with 160EU vaccine given IM, the monkeys were boosted after 1 month with 160EU vaccine given IM. After the booster, most of the monkeys sero-converted and developed EV71 neutralizing antibodies. Importantly however, following a sham challenge with the live virus strain FY23 given 4 weeks after immunization, the neutralizing antibody titers rose rapidly indicating a vigorous anamnestic response. Based on the neutralizing antibody response following the vaccination and the extent of anamnestic response generated in the immunized monkeys, vaccination group was superior to mock group. This study indicates that the EV71 candidate vaccine is capable of generating protective levels of EV71 neutralizing antibodies in neunatal rhesus monkeys and primes the immune system effectively against a subsequent exposure to EV71.

关 键 词：肠道病毒71型 (Enterovirus 71 EV71) 灭活疫苗 免疫原性 恒河猴婴猴 

分 类 号：R392[医药卫生—免疫学]