左卡尼汀对大鼠肾缺血再灌注损伤时能量代谢的影响  

Effect of L-carnitine on energy metabolism during renal ischemic reperfusion injury in rats

作　　者：许益笑[1] 王德选[2] 卢立肖[2] 杨宇真[2] 杨青[2] 王丽[3] 熊锡山[3] 

机构地区：[1]温州医学院病理生理学教研室,浙江温州325035 [2]温州医学院附属育英儿童医院,肾内科325027 [3]上海长征医院肾脏病研究所,上海200003

出　　处：《温州医学院学报》2011年第3期220-223,共4页Journal of Wenzhou Medical College

摘　　要：目的:探讨左卡尼汀对肾缺血再灌注损伤(RIRI)时能量代谢的影响及其机制。方法:健康SD大鼠48只,随机分为对照组和左卡尼汀治疗组(治疗组),组内再分为假手术组及再灌注1 h(IR1h)、6 h(IR6h)、12 h(IR12h)组(n=6)。建立大鼠肾急性缺血再灌注模型。检测大鼠血清尿素氮(Bun)、肌酐(Scr)水平和组织游离脂肪酸(FFA)含量、钠钾ATP酶活力,并观察肾组织超微结构变化。结果:对照组IR6h、IR12h组大鼠的BUN、Scr较假手术组显著性升高(P<0.05或P<0.01);IR12h组大鼠肾组织FFA含量较假手术组显著升高(P<0.01);IR6h、IR12h组钠钾ATP酶活力均显著下降(P<0.05或P<0.01)。治疗组IR12h组的BUN和IR6h、IR12h组的Scr低于同时点对照组(均P<0.01);IR12h组钠钾ATP酶活力显著高于而FFA含量显著低于同时点对照组(均P<0.01)。超微结构显示,治疗组肾小管上皮细胞线粒体损伤明显减轻。结论:左卡尼汀对IRIR具有保护作用,其机制与促进脂肪酸β氧化、提高钠钾ATP酶活力、优化能量代谢有关。Objective： To probe the effect of L-carnitine on energy metabolism during renal ischemic reperfusion injury and the possible mechanisms.Methods： Model of renal ischemia-reperfusion was established,and 48 SD healthy rats were divided into untreated control and L-carnitine treated groups.Within each group,rats were further divided into four subgroups,Sham,and 1 h,6 h and 12 h IR group（n =6）.The levels of blood serum urea nitrogen（Bun）and creatinine（Scr）,the free fatty acid（FFA）content and Na＋-K＋-ATPase activity in nephritic tissues were measured.The ultrastructural changes in renal tissue were observed under electron microscope.Results： In control groups,compared with those of the sham operation group,Scr and Bun levels at IR6h and IR12h groups increased significantly（P 0.05 or P 0.01）;the FFA content in nephritic tissues at IR12h group went up markedly（P 0.01） while the Na＋-K＋-ATPase activity of IR6h and IR12h groups declined obviously（P 0.05 or P 0.01）.As to the L-carnitine treatment groups,compared with those of the control group rats with the same reperfusion time,the Bun levels of 12 h group and the Scr level of IR6h,12h groups were obviously lower（each P 0.01）;the FFA content in nephritic tissues at IR12h group was markedly lower while the Na＋-K＋-ATPase activity was obviously higher（each P 0.01）.In addition,ultrastructure indicated injuries of the mitochondriarenal in tubular epithelial cell were attenuated after L-carnitine administration.Conclusion： L-carnitine protects kidney against acute renal ischemia reperfusion injury through promotion of β-oxidation of fatty acids,increasing Na＋-K＋ ATPase activity,and finally grading-up energy metabolism.

关 键 词：左卡尼汀 肾 缺血再灌注 游离脂肪酸 钠钾ATP酶 

分 类 号：R692.5[医药卫生—泌尿科学]