鸭肠炎病毒gB胞浆区基因片段的克隆及原核表达  

Cloning and prokaryotic expression of cytoplasmic domain of gB gene of duck enteritis virus

作　　者：马波[1] 李洪涛[2] 刘峰源[1] 张扬[1] 刘晓玫[1] 乌伊罕[1] 王君伟[1] 

机构地区：[1]东北农业大学动物医学学院,黑龙江哈尔滨150030 [2]东北农业大学资源与环境学院,黑龙江哈尔滨150030

出　　处：《中国兽医科学》2011年第6期607-609,共3页Chinese Veterinary Science

基　　金：东北农业大学博士启动基金项目;黑龙江省教育厅重大科技项目(10541Z004)

摘　　要：为了研究鸭肠炎病毒gB胞浆区的反应原性,以鸭肠炎病毒C-KCE株为模板,扩增了gB胞浆区基因片段,并构建了其原核表达载体pET-32a-gB-N,将其转化至大肠杆菌感受态细胞Rosetta(DE3)pLysS中,经IPTG诱导,用SDS-PAGE及Western-blot检测目的蛋白的表达情况及其反应原性。SDS-PAGE分析表明获得了30ku的融合蛋白,在Western-blot检测中,纯化的融合蛋白可与鸭肠炎病毒阳性血清发生特异性反应,表明其具有良好的反应原性。In order to identify the reactinogenicity of gB cytoplasmic domain of duck enteritis virus（DEV）,the interested gene fragment was amplified from the genome of DEV C-KCE strain.The recombinant prokaryotic expression vector pET-32a-gB-N was constructed,and the recombinant plasmid was transformed into Escherichia coli Rosetta（DE3） pLysS.The expressed protein was analyzed by SDS-PAGE,and the reactinogenicity of the purified protein was detected by Western-blot.In result,the expressed fusion protein was 30 ku in size.The purified fusion protein could react with DEV positive serum specifically,indicating that the purified protein had good reactinogenicity.

关 键 词：鸭肠炎病毒 gB胞浆区 反应原性 

分 类 号：S852.659.1[农业科学—基础兽医学]