油茶芽苗砧嫁接口愈合过程中苯丙烷代谢的若干生理指标  

Some physiological phenylpropanoid metabolic characters in developing nurse seed grafting union of Camellia oleifera

作　　者：冯金玲[1] 杨志坚[2] 陈世品[2] 刘玉宝[2] 陈辉[1,2] 

机构地区：[1]福建农林大学作物学院,福建福州350002 [2]福建农林大学林学院,福建福州350002

出　　处：《福建农林大学学报（自然科学版）》2011年第3期264-270,共7页Journal of Fujian Agriculture and Forestry University:Natural Science Edition

基　　金：国家林业局科技支撑计划项目(2006BAD18B0204-FJ);福建省科技厅重大项目(2006NZ0001-1;2006N0007)

摘　　要：为探讨油茶芽苗砧嫁接口和对照实生苗愈伤口的发育差异,在处理后的2-55 d对嫁接口和愈伤口间隔取样,检测苯丙烷代谢若干生理指标.结果表明:嫁接口的苯丙氨酸解氨酶(PAL)活性极显著低于愈伤口;过氧化物酶(POD)活性在嫁接后的第14-35天极显著高于愈伤口;苗木重量、纤维素含量、绿原酸含量、多酚氧化酶(PPO)活性与愈伤口无显著差异;各生理指标变化的拐点数量和所处时间与愈伤口不同.相关分析表明:嫁接口的PPO活性与苗木重量,绿原酸含量与纤维素含量,绿原酸含量与苗木重量之间的相关性与愈伤口相当;绿原酸含量与PPO活性,绿原酸含量与POD活性,纤维素含量与苗木重量之间的相关性明显高于愈伤口;而PPO活性与POD活性,PPO活性与纤维素含量之间的相关性却低于愈伤口.To discuss the difference of development between the nurse seed grafting union in Camellia oleifera and the healing wound,the test materials were sampled with interval in 2-55 days after treatment,and some physiological phenylpropanoid metabolic indices were measured.The phenylalanine ammonialyase（PAL） activities of the grafting union were highly significantly lower than the healing wound.During 14-35 days after treatment,the peroxidase（POD） activities of the grafting union were highly significantly higher.Compared with the healing wound,the weight of seedling,the content of cellulose,the content of chlorogenic acid and the polyphenoloxidase（PPO） activities of the grafting union had no significant difference.The number and the time of break point in the grafting union physiological indices differentiated from the healing wound.The correlations between PPO activities and the weight of seedling of the grafting union,the content of chlorogenic acid and the content of cellulose,the content of chlorogenic acid and the weight of seedling were equivalent with the healing wound.The correlations between the content of chlorogenic acid and the PPO activities,the content of chlorogenic acid and the POD activities,the content of cellulose and the weight of seedling were obviously higher than that of the healing wound.But the correlations between the PPO activities and the POD activities,the PPO activities and the content of cellulose were lower than that of the healing wound.

关 键 词：油茶 芽苗砧嫁接 苯丙烷代谢 愈伤口 生理指标 

分 类 号：S565.9[农业科学—作物学]