硬粒小麦与野生二粒小麦重组自交系群体穗部性状的QTL定位  

QTL Mapping of Spike Traits in Population of Recombinant Inbred Lines between Durum Wheat×Wild Emmer Wheat

作　　者：严俊[1,2,3] 张玲玲[1] 万兵[1] 苟君波[1] 王运长[1] 许昌敏[1] Tzion Fahima 程剑平[2] 

机构地区：[1]贵州大学生命科学院,贵阳550025 [2]贵州大学农学院,贵阳550025 [3]海法大学进化研究所,以色列海法31905

出　　处：《四川农业大学学报》2011年第2期147-153,共7页Journal of Sichuan Agricultural University

基　　金：贵州省"十一五"重点攻关项目[黔科合NY(2006)3002F];贵州省优秀青年科技人才培养计划(2006-2011)

摘　　要：野生二粒小麦是现代栽培小麦的祖先种,含有极为丰富的遗传多样性。本研究利用来自以色列Gitit的野生二粒小麦G18-16与来自欧洲的硬粒小麦栽培品种Langdon杂交构建的重组自交系F6代152个家系,进行了抽穗期、单株有效穗数、穗粒数、穗长、芒长等数量性状基因位点（QTL）分析,发现全部家系在5个性状上表现出宽广的遗传差异。14个穗部性状加性QTL被定位,LOD值为1.9~13.4,贡献率为7.3%~54.2%。控制抽穗期的QTL共3个,定位在3A（2个）和7B上;在2A（2个）和5A上共找到3个控制穗粒数的QTL;在5B上找到2个控制单株有效穗数的QTL;控制穗长的3个QTL分布在5A（2个）和7A上;在4B,5A和7A上共找到3个控制芒长的QTL。获得的QTL可用于今后分子标记辅助育种改良现代栽培小麦。Wild emmer wheat,Triticum turgidum L.ssp.dicoccoides,is the progenitor of modern tetraploid and hexaploid cultivated wheats,whose genepool harbor a rich allelic repertoire for various traits.In this study,the genetic and phenotypic basis of spike traits,e.g.heading stage（HS）,spike number per plant（SPP）,kernel number per spike（KNS）,spike length（SL）,and awn length（AL） were analyzed in tetraploid wheat population consisting of 152 F6 recombinant inbreed lines（RILs） derived from a cross between T.dicoccoides（G18-16） and durum wheat cultivar（Langdon）.Wide genetic variation was found among RILs for all studied traits.A total of 14 QTL effects associated with five spike traits were detected,with a LOD score range of ca.1.9 to 13.4,explaining phenotypic variation 7.3%~54.2%.The identified QTLs included： three QTLs for HS located on chromosomes 3A（2 QTLs） and 7B;three QTLs for KNS,located on chromosomes 2A（2 QTLs） and 5A;two QTLs for SPP,located on chromosome 5B（2 QTLs）;three QTLs for SL,located chromosomes 5A（2 QTLs） and 7A;three QTLs for AL,located chromosomes 4B,5A and 7A,respectively.The identified QTLs could be used to the future marker-assisted selection programs,facilitating the use of wild alleles for yield enhancement in elite wheat cultivars.

关 键 词：野生二粒小麦 硬粒小麦 重组自交系 穗部性状 数量性状基因位点 

分 类 号：S512.1[农业科学—作物学]