完整顶体精子注入卵细胞后对胚胎发育的影响  

A Study of Spermatozoa with Acrosome on Embryo Development

作　　者：梁鑫[1] 单旭东[1] 蒋小辉[1] 漆著[1] 孟衍建[1] 

机构地区：[1]成都中医药大学第二附属医院生殖中心,成都610041

出　　处：《四川农业大学学报》2011年第2期245-248,共4页Journal of Sichuan Agricultural University

基　　金：成都中医药大学校基金(ZRYB200814)

摘　　要：分别注射1、3、5个顶体完整的人精子于金黄地鼠卵中,对照组同时注射数量相等的无顶体精子,观察卵活化、受精及后期发育情况。结果发现随着注入精子数量的增加,卵发育的异常率显著增加,而注射无顶体精子组,无论注射精子数量为多少,均无异常发生。此外,注射多个精子于金黄地鼠卵中时,多原核(PN)形成率顶体完整组明显低于无顶体组(P<0.01)。实验表明完整顶体的精子所含顶体酶等对卵及后期胚胎发育可能存在一定的影响,行卵胞浆内精子显微注射(ICSI)时,推测注射无顶体精子更合理,应能增加该技术的有效性。In this study,1,3 or 5 human spermatozoa with acrosomes injected into a hamster oocyte in experimental group,simultaneously,the same number spermatozoa without their acrosomes injected into control group hamster oocyte,oocytes activation,fertilization and post-development were observed.The results showed that the abnormal rate of oocytes development were significantly increased with the increasing of the number of spermatozoa injection.However,no matter how many number of spermatozoa injection,there were no abnormal oocytes in control group.In addition,when more human spermatozoa were injected into individual hamster oocytes,the multi-pronuclear formation rate of acrosomal integrity group were significantly lower than that of control group（P0.01）.Experimental results showed that spermatozoa acrosomal enzymes may have some influence on the eggs and embryonic post-development.The spermatozoa of removal acrosomes were more preferable and effective before ICSI.

关 键 词：精子 顶体酶 卵胞浆内单精子注射术 

分 类 号：S865.140.3[农业科学—野生动物驯养]