白及不同提取部位对小鼠止血活性实验  

Effects of diferent extracts from Bletilla colloid on hemostasis activity in mice

作　　者：吴久健[1] 孟岳良[2] 邹俪华 邱彦[4] 

机构地区：[1]解放军第37医院,四川雅安625000 [2]成都军区联勤部卫生部,四川成都610015 [3]总参管理保障部保健处,北京100034 [4]第二军医大学药学院,上海200433

出　　处：《药学实践杂志》2011年第3期206-207,234,共3页Journal of Pharmaceutical Practice

摘　　要：目的确定白及的止血活性部位,为其止血活性成分的筛选提供线索和依据。方法用70%乙醇(EtOH)对白及原药材进行渗漉提取,然后,依次用石油醚(Pet)、乙酸乙酯(EtOAc)、正丁醇(n-BuOH)对醇浸膏进行萃取,分别制得Pet部分、EtOAc部分、n-BuOH部分和H2O部分,通过测定小鼠凝血时间和出血时间,考察其活性部位。结果 n-BuOH部分和H2O部分具有止血作用,而EtOAc部分具有延长凝血、出血时间作用。结论白及中具有止血作用的活性成分主要存在于n-BuOH部分和H2O部分,而EtOAc部分具有活血作用。Objective To determine the active position of Bletilla colloid and offer references to the seleciton of its active composition.Methonds The crude Bletilla colloid was extracted by 70% ethanol permeating,then the petroleum ether extract,ethyl acetate exctract,n-butyl alcohol extract and water extract were exctracted from alcohol extract in turn by petroleum,ethyl acetate,n-butyl alcohol extraction.The active position was determined by measuring the time of mice blood coagulation and hemorrhage.Results N-butanol extract and water extract from Bletilla colloid had hemostasis effect,ethyl acetate extract had the effect of prolonging the time of blood coagulation and hemorrhage.Conclusion N-butanol extract and water extract from Bletilla colloid was major active extracts played a part in hemostasis,ethyl acetate extract had the effect of invigoration the circulation of blood.

关 键 词：白及 出血 止血 小鼠 活性 

分 类 号：R965.15[医药卫生—药理学]