组织胺针型生物传感器的研制及在体应用  

New Needle-type Histamine Biosensor and Its Application In Vivo

作　　者：林志红[1] 柳再华[1] 吴蒙[1] 俞凤兰 任恕 

机构地区：[1]同济医科大学基础医学院 化学教研室,武汉430030

出　　处：《化学传感器》1999年第4期38-41,共4页Chemical Sensors

基　　金：国家攀登计划资助(JL93010)

摘　　要：本文研制了一种能用于经络研究的生物传感针。该针能直接插入确定的组织中在体(in vivo)测定组织胺含量的动态变化过程。它是用高分子材料修饰已预处理好的针灸针,然后通过小牛血清和戊二醛将组织胺氧化酶交联其上。本文讨论了pH、温度等对该传感针的影响,其线性范围为:6.0×10^(-6)—3.1×10^(-3)mol/L,检出下线为2.4×10^(-6)mol/L。响应时间为3min。We have made a kind of biosensor for the research of acupuncture, which could be insert into tissue of animals to detect directly the change process of the concentration of histamine in vivo. This biosensor was modified by a polymer material. Histaminese was bind in polymer membrane with bovine serum albumin and glutaraldchyydc. The influences of pH, temperature, are discussed. It can determine the substrates with the linear range 6.0×106-3. 1×10 3mol/L, the detection of limit is 2. 4×10 6mol/L,responsed time is about 3 min.

关 键 词：针灸型 传感针 组织胺 生物传感器 

分 类 号：Q811.4[生物学—生物工程]