大豆异黄酮对卵巢切除大鼠糖脂代谢和血清瘦素、抵抗素表达的影响  

Effects of genistein on glucose and lipid metabolism and the expression of serum leptin and resistin in ovariectomized SD rats

作　　者：龚华芳[1] 张亚兴[1] 王晓东[1] 曾耀英[1] 

机构地区：[1]暨南大学生命科学技术学院组织移植与免疫实验中心,广东广州510632

出　　处：《暨南大学学报（自然科学与医学版）》2011年第3期324-327,338,共5页Journal of Jinan University(Natural Science & Medicine Edition)

基　　金："973"国家重点基础研究项目(2006CB504201;2004CB720100)

摘　　要：为探讨大豆异黄酮对卵巢切除大鼠糖脂代谢和血清瘦素、抵抗素表达的影响,将12周SD大鼠分成3组,假手术(Sham)组、卵巢切除(OVX)组和大豆异黄酮(OVX+Gen)组,每组7只,卵巢切除术后10 d,OVX组和Sham组大鼠每天腹腔注射生理盐水0.1 ml/只,OVX+Gen组每天注射大豆异黄酮0.2 mg/只,连续注射30 d后处死取血,检测子宫病理改变、血糖血脂和胰岛素水平、血清瘦素和抵抗素含量.结果发现卵巢切除后大鼠总胆固醇、HDL和LDL均增加(P<0.05),但胰岛素、血糖、甘油三酯、血清瘦素和抵抗素水平变化不明显(P>0.05);卵巢切除后补充大豆异黄酮,可促进子宫内膜上皮部分增生,但胰岛素、血糖、总胆固醇、甘油三酯、LDL和HDL、血清瘦素和抵抗素水平均无明显变化(与OVX组相比,P>0.05),结果提示大豆异黄酮对卵巢切除大鼠糖脂代谢参数和血清瘦素、抵抗素水平影响不大.To delineate the effects of ovariectomy （0VX） and genistein cose, insulin, total cholesterol, LDL, HDL, triglycerides, serum leptin treatment on the levels of gluand resistin, female SD rats （12-week old） were divided into three groups, control （Sham）, OVX and OVX treated with genistein （OVX ＋ Gen） （n =7, each group）. After operation for 10 days, rats were treated with genistein （ip, 0. 2 mg per day） （ OVX ＋ Gen） and vehicle （ Sham and OVX rats） for 30 days. The pathological conditions of the uterus, and the levels of glucose, total cholesterol, LDL, HDL, triglycerides, serum leptin and resistin were analyzed. The results indicated that OVX upregulated the levels of total cholesterol, LDL and HDL, but showed no effect on the levels of insulin, blood glucose, triglyceride, serum leptin and resistin. Although the treatment with genistein did not change the levels of insulin, blood glucose, total cholesterol, triglyceride, LDL, HDL, serum leptin and resistin, it stimulated the proliferation of en- dometrial epithelial cells. The results suggest that genistein could promote the proliferation of endometrial epithelial cells, but show no effects of on the glucose and lipid metabolism, and the levels of serum leptin and resistin in ovariectomized female rats.

关 键 词：卵巢切除 大豆异黄酮 血糖 血脂 瘦素 抵抗素 

分 类 号：Q95[生物学—动物学] R3[医药卫生—基础医学]