基于免疫组织化学方法的中华蜜蜂蕈形体胚后发育过程中细胞增殖和凋亡的观察  

Observation of neurogenesis and programmed cell death in the mushroom bodies of the honeybee,Apis cerana cerana (Hymenoptera:Apidae),using immunohistochemistry method

作　　者：李兆英[1,2] 奚耕思[2] 

机构地区：[1]陕西教育学院生物科学与技术系,西安710061 [2]陕西师范大学生命科学学院,西安710062

出　　处：《昆虫学报》2011年第6期657-663,共7页Acta Entomologica Sinica

基　　金：陕西省科技计划项目(2008K02211)

摘　　要：蕈形体是昆虫学习和其他复杂行为的整合中心。本研究通过形态解剖、BrdU免疫组织化学和原位末端转移酶标记(TUNEL)细胞凋亡检测等技术,对中华蜜蜂Apis cerana cerana蕈形体胚后发育过程中细胞的增殖和凋亡模式进行了比较研究。结果表明:中华蜜蜂的蕈形体起源自幼虫早期脑背侧的几个大型的成神经细胞,它们通过不对称的细胞分裂产生成神经细胞,随后这些细胞经过多次对称分裂形成中增殖细胞群和侧增殖细胞群,最终生成了所有的Kenyon细胞。蕈形体的蕈体柄出现在3龄幼虫;蕈体冠体积的迅速增加发生在蛹期。蕈形体发育过程中,细胞凋亡主要集中在蛹发育期的3-6d内,细胞凋亡的开始和细胞增殖的终止在时间和空间上非常一致。本研究为蜜蜂学习记忆等行为学研究提供了理论依据。Mushroom bodies （MBs） are insect brain centers involved in learning and other complex behaviors.The proliferation and programmed cell death （PCD） pattern of MBs in the honeybee,Apis cerana cerana,was comparatively studied by using 5-bromo-2-deoxyuridine （BrdU） incorporation and the terminal deoxynucleotidyl transferase-mediated dUTP nick-end labelling （TUNEL） technique.The results showed that the MBs are created in their entirety by several neuroblasts per hemisphere.Asymmetric divisions produce a smaller ganglion mother cell （GMC） and regenerate the neuroblast.Kenyon cells in a proliferation cluster are formed through symmetrical division of MB neuroblasts.These neuroblasts divide continuously from early embryogenesis until the late pupal stage.Peduncular neuropil was first visible in the 3rd instar.The calycal neuropil rapidly increased in size during the pupal stage.Extensive apoptosis in the MBs could be detected only within a narrow time window from day 3 to day 6 of the pupal stage during metamorphosis.Extensive apoptosis in the MB proliferative clusters started at about the time when proliferation began to cease.This study provides a theoretical basis for behavioural research.

关 键 词：中华蜜蜂 蕈形体 胚后发育 增殖 凋亡 5-溴脱氧尿嘧啶核苷 原位末端转移酶标记 

分 类 号：Q965[生物学—昆虫学]