环氧苍耳素Ⅰ对菜青虫中肠消化酶和羧酸酯酶活性的影响  

Effect of epoxyxanthatin Ⅰ from Xanthium sibiricum (Asteraceae) on the activity of midgut digestive enzymes and carboxylesterase in Pieris rapae (Lepidoptera:Pieridae) larvae

作　　者：周琼[1] 魏美才[2] 欧晓明[3] 

机构地区：[1]湖南师范大学生命科学学院,长沙410081 [2]中南林业科技大学昆虫系统与进化生物学实验室,长沙410004 [3]湖南化工研究院国家农药创制工程技术研究中心,长沙410007

出　　处：《昆虫学报》2011年第6期729-733,共5页Acta Entomologica Sinica

基　　金：国家自然科学基金项目(30970421);湖南省自然科学基金重点项目(06JJ20099);湖南省教育厅重点科研项目(10A077);湖南师范大学化学生物学及中药分析教育部重点实验室开放基金(KLCBTCMR2009-12)

摘　　要：【目的】探明苍耳Xanthium sibiricum活性物质——环氧苍耳素Ⅰ对菜青虫的作用机理。【方法】采用饲喂法处理4龄菜青虫Pieris rapae,测试从苍耳中分离提纯的环氧苍耳素Ⅰ(为倍半萜内酯类物质)对菜青虫中肠蛋白酶、淀粉酶和羧酸酯酶活性的影响。【结果】环氧苍耳素Ⅰ对蛋白酶活性的抑制最强,处理后12,24和48h,菜青虫中肠蛋白酶抑制率分别为20.95%,29.38%和50.06%;其次为淀粉酶,抑制率分别为11.89%,39.01%和31.92%。同时,中肠羧酸酯酶活性在处理后12h与对照之间无显著变化,24h时活性被显著抑制,而48h时活性却明显高于对照。【结论】环氧苍耳素Ⅰ对昆虫中肠消化酶活性的抑制,可能是引起昆虫表现取食抑制和生长发育不良的重要原因之一。[Aim] This study aims to reveal the mechanism of epoxyxanthatin Ⅰ （sesquiterpe lactone from Xanthium sibiricum） on Pieris rapae larvae.[Methods] The activity of midgut protease,amylase and carboxylesterase in P.rapae larvae were investigated by feeding to 4th larvae of P.rapae.[Results] Epoxyxanthatin Ⅰ showed the most obvious inhibition on midgut protease,whose activities were inhibited 20.95%,29.38% and 50.06% at 12 h,24 h and 48 h after treatment respectively,while it showed the less obvious inhibition on midgut amylase,whose activities were inhibited 11.89%,39.01% and 31.92%,respectively.Meanwhile,no significant differences in carboxylesterase activity were detected between the controls and the epoxyxanthatin Ⅰ treated samples at 12 h after treatment,while the activity was obviously inhibited at 24 h after treatment and significantly increased at 48 h after treatment compared with that of the control.[Conclusion] The results suggest that the inhibition effect of epoxyxanthatin Ⅰ on midgut digestive enzymes of insects may be one of the causes to inhibit feeding and growth of insects.

关 键 词：菜青虫 苍耳 环氧苍耳素Ⅰ 中肠 消化酶 羧酸酯酶 酶活性 

分 类 号：Q965[生物学—昆虫学]