猪附红细胞体感染小鼠血清IgG和IgE水平的变化  

Serum IgG and IgE Levels of Mice Infected with Mycoplasma suis

作　　者：巴彩凤[1] 谢建中 宋立先 

机构地区：[1]辽宁医学院实验动物中心,辽宁锦州121001 [2]山东省庆云县人民医院,山东庆云253700

出　　处：《中国生物制品学杂志》2011年第6期679-681,共3页Chinese Journal of Biologicals

基　　金：辽宁省科技基金资助项目(2006408002-4)

摘　　要：目的建立猪附红细胞体感染BALB/c小鼠模型,检测小鼠血清IgG和IgE水平的变化。方法将未感染猪附红细胞体的BALB/c小鼠随机分为3组:A组注射猪附红细胞体阳性血液,B组注射猪附红细胞体阴性血液,C组注射生理盐水。采用镜检和PCR法检测各组小鼠猪附红细胞体感染情况;ELISA法检测各组小鼠血清IgG和IgE水平。结果镜检观察A组小鼠血液中可见猪附红细胞体。PCR结果显示,A组小鼠血液中可扩增出602 bp的特异性条带。在接种后第7和19天,3组小鼠血清IgG水平差异无统计学意义(P>0.05),至第10、13和16天,A组小鼠血清IgG水平明显高于B组和C组(P<0.01),B组明显高于C组(P<0.01);在接种后第10、13和22天,3组小鼠血清IgE水平差异无统计学意义(P>0.05),至第16和19天,A组小鼠血清IgE水平明显高于B组和C组(P<0.01),B组明显高于C组(P<0.01)。结论已成功建立猪附红细胞体感染小鼠模型,IgG和IgE在小鼠抗猪附红细胞体免疫应答中发挥重要作用。Objective To establish BALB/c mouse model of Mycoplasma suis infection and observe the changes of serum IgG and IgE levels.Methods BALB/c mice uninfected with M.suis were divided into groups A,B and C,and injected with M.suis-positive blood,M.suis-negative blood and physiological saline respectively.The mice in various groups were determined for M.suis infection by microscopy and PCR,and for serum IgG and IgE levels by ELISA.Results M.suis was observed in blood of mice in group A by microscopy.Specific band at a length of 602 bp was amplified from blood of mice in group A by PCR.The serum IgG levels of mice in the three groups showed no significant difference on days 7 and 19 after injection（P 0.05）,while were significantly higher in group A than in groups B and C（P 0.01）,and in group B than in group C（P 0.01）,on days 10,13 and 16.However,the serum IgE levels of mice in the three groups showed no significant difference on days 10,13 and 22（P 0.05）,while were significantly higher in group A than in groups B and C（P 0.01）,and in group B than in group C（P 0.01） on days 16 and 19.Conclusion The mouse model of M.suis infection was successfully established.IgG and IgE played important roles in immune response against M.suis.

关 键 词：支原体属 感染 小鼠 模型 动物 免疫球蛋白G 免疫球蛋白E 

分 类 号：R392-33[医药卫生—免疫学]