重组弓形虫热休克蛋白70鼻内及皮下免疫小鼠诱导的免疫应答比较  

Immune Responses Induced with Recombinant Toxoplasma gondii Heat Shock Protein 70 by Intranasal and Subcutaneous Routes in Mice

作　　者：殷丽天[1] 曹蕾[2] 王海龙[2] 孟晓丽[2] 张建红[2] 殷国荣[2] 

机构地区：[1]山西医科大学生理学系细胞生理学省部共建教育部重点实验室,太原030001 [2]山西医科大学医学寄生虫学研究所寄生虫学教研室,太原030001

出　　处：《中国生物制品学杂志》2011年第6期689-692,共4页Chinese Journal of Biologicals

基　　金：国家自然科学基金(81071374)

摘　　要：目的比较重组弓形虫热休克蛋白70(rTgHSP70)滴鼻及皮下免疫小鼠诱导的免疫应答及其持续时间。方法将90只BALB/c小鼠随机分为3组:滴鼻组用20μg rTgHSP70滴鼻,皮下组用80μg rTgHSP70皮下免疫,间隔2周加强免疫1次,对照组不处理。末次免疫后第1、2、3、4、5、6周,每组分别处死小鼠5只,ELISA法检测血清IgG水平、鼻咽和肠冲洗液sIgA水平;分离脾淋巴细胞和肠上皮内淋巴细胞(IEL)并计数。结果滴鼻组小鼠血清IgG水平第1～6周高于皮下组和对照组,其中第3～5周差异有统计学意义(P<0.001);皮下组小鼠第3、4周显著高于对照组(P<0.001)。滴鼻组小鼠第3～5周,皮下组小鼠第4～5周鼻咽冲洗液sIgA水平显著高于对照组(P<0.001)。滴鼻组小鼠小肠冲洗液sIgA水平第1～6周显著高于皮下组和对照组(P均<0.001);皮下组小鼠第3、4周显著高于对照组(P<0.001)。滴鼻组小鼠脾淋巴细胞数量第2～4周显著高于对照组(P<0.000 1),3～4周显著高于皮下组(P<0.001);皮下组小鼠第2～4周显著高于对照组(P<0.001)。滴鼻组小鼠IEL数量第1～6周显著高于对照组(P<0.000 1),第3～4周显著高于皮下组(P<0.000 1),皮下组小鼠第1～5周显著高于对照组(P<0.05)。结论 rTgHSP70皮下和滴鼻免疫小鼠均能诱导有效的黏膜和系统免疫应答,且滴鼻免疫的效果优于皮下免疫,是rTgHSP70更为有效的免疫途径。Objective To compare the immune responses and their persistence induced with recombinant Toxoplasma gondii heat shock protein 70（rTgHSP70） in mice by intranasal and subcutaneous routes.Methods Ninety 5-week-old BALB/c mice were randomly divided into 3 groups.The mice in intranasal group were immunized twice with 20 μg of rTgHSP70 by intranasal drip,and those in subcutaneous group with 80 μg of rTgHSP70 by subcutaneous injection,at an internal of 2 weeks,while those in control group were unimmunized.Five mice in each group were killed respectively at weeks 1,2,3,4,5 and 6 after the last immunization and determined for IgG levels in sera and sIgA levels in nasopharynx and intestinal washes by ELISA,of which the spleen lymphocytes and intraepithelial lymphocytes（IELs） were isolated and counted.Results The IgG levels in sera of mice at weeks 1 ~ 6 after the last immunization were higher,while those at weeks 3 ~ 5 were significantly higher in intranasal group than in subcutaneous and control groups（P 0.001）,and those at weeks 3 ~ 4 were significantly higher in subcutaneous group than in control group（P 0.001）.The sIgA levels in nasopharynx washes of mice in intranasal group at weeks 3 ~ 5 as well as those in subcutaneous group at weeks 4 ~ 5 were significantly higher than those in control group（P 0.001）.The sIgA levels in intestinal washes of mice at weeks 1 ~ 6 were significantly higher in intranasal group than in subcutaneous and control groups（P 0.001）,while those at weeks 3 ~ 4 were significantly higher in subcutaneous group than in control group（P 0.001）.The spleen lymphocyte counts were significantly higher in intranasal group than in control group at weeks 2 ~ 4（P 0.000 1）,in intranasal group than in subcutaneous group at weeks 3 ~ 4,and in subcutaneous group than in control group at weeks 2 ~ 4（P 0.001）.The IEL counts were significantly higher in intranasal group than in control group at weeks 1 ~ 6（P 0.000 1）,in intranasal group than in subcutaneous group at weeks 3 ~

关 键 词：刚地弓形虫 热休克蛋白70 重组蛋白 鼻内免疫 

分 类 号：R382.5[医药卫生—医学寄生虫学] R392-33[医药卫生—基础医学]