Chemiluminescence determination of melamine with Luminol-K_3Fe(CN)_6 system  

作　　者：Xiao- Shuang Tang Xi-Yan Shi Yu-Hai Tang Zhong-Jin Yue Qi-Qi He 

机构地区：[1]The Second Affiliated Hospital, Lanzhou University, Lanzhou 730030, China [2]Institute of Analytical Sciences, Xi'an Jiaotong University. Xi'an 710061, China.

出　　处：《Journal of Pharmaceutical Analysis》2011年第2期104-107,共4页药物分析学报（英文版）

摘　　要：A sensitive chemiluminescence（CL） method was developed for determining melamine in urine and plasma samples based on the fact that melamine can remarkably enhance the chemiluminescence of Luminol-K3 Fe（CN）6 system in alkaline medium. The determination conditions were optimized. Under optimum conditions, the chemiluminescence intensity had a good linear relationship with melamine in the range of 9.0 × 10^-9 - 7.0 × 10^-6 g/mL with a correlation coefficient of 0. 9992. The detection limits （3o） were 3.54 ng/mL for urine sample and 6.58 ng/mL for plasma sample. The average recoveries of melamine were 102.6% for urine sample and 95.1% for plasma sample. Melamine in samples was extracted with liquid-liquid extraction procedures and the assay results coincided very well with that determined with flow injection chemiluminescence method. The method provides a reproducible and stable approach for sensitive detection and quantification of melamine in urine and plasma samples.A sensitive chemiluminescence（CL） method was developed for determining melamine in urine and plasma samples based on the fact that melamine can remarkably enhance the chemiluminescence of Luminol-K3 Fe（CN）6 system in alkaline medium. The determination conditions were optimized. Under optimum conditions, the chemiluminescence intensity had a good linear relationship with melamine in the range of 9.0 × 10^-9 - 7.0 × 10^-6 g/mL with a correlation coefficient of 0. 9992. The detection limits （3o） were 3.54 ng/mL for urine sample and 6.58 ng/mL for plasma sample. The average recoveries of melamine were 102.6% for urine sample and 95.1% for plasma sample. Melamine in samples was extracted with liquid-liquid extraction procedures and the assay results coincided very well with that determined with flow injection chemiluminescence method. The method provides a reproducible and stable approach for sensitive detection and quantification of melamine in urine and plasma samples.

关 键 词：CHEMILUMINESCENCE flow injection MELAMINE URINE PLASMA 

分 类 号：O657.3[理学—分析化学] TQ226.62[理学—化学]