融合智能检测的DNA序列预处理新方法  

Novel approach for DNA sequence preprocessing with intelligent detection

作　　者：刘君[1,2] 熊忠阳[1] 王银辉[1] 

机构地区：[1]重庆大学计算机学院,重庆400044 [2]重庆广播电视大学理工学院,重庆400052

出　　处：《重庆大学学报（自然科学版）》2011年第6期132-136,共5页Journal of Chongqing University

基　　金：中国博士后科学基金资助项目(20070420711);重庆市科委自然科学基金计划资助项目(2007BB2372)

摘　　要：提出一种融合智能检测的DNA序列预处理新方法。该方法不需要预先给出载体序列、剪接位点和克隆适配片段等信息,通过统计分析、随机搜索和构建图操作等方法自动发现并定位垃圾信息。以Zebrafish DNA序列为样本进行的预处理实验结果证明该方法能够显著提高DNA序列预处理的效率和准确性,在处理超长序列时更稳定、错误率更低。A novel approach for DNA sequence preprocessing by merging intelligent detection is proposed. This approach can automatically find and locate contaminants using statistical analysis methods, random search and graph-theoretic operations, while no extra background information, such as vector sequence, splice site and clone adapter are needed during preprocessing. Experiments on Zebrafish DNA show that the approach can significantly improve the efficiency and accuracy of DNA sequence preprocessing and provide more stable performance than the conventional methods do, particularly in high-throughput DNA sequence preprocessing.

关 键 词：DNA序列 预处理 智能检测 载体 污染清除 

分 类 号：TP301[自动化与计算机技术—计算机系统结构]