片仔癀体外抗肿瘤作用研究  

Study on antitumor effect of Pien Tze Huang in vitro

作　　者：郭珮蕾[1] 刘吉华[1] 余伯阳[1] 

机构地区：[1]中国药科大学中药复方研究室,江苏南京211198

出　　处：《药物评价研究》2011年第3期171-173,共3页Drug Evaluation Research

基　　金：国家支撑计划项目(2007BAI36B01);江苏省"六大人才高峰"资助项目(第五批次)

摘　　要：目的研究片仔癀对体外肿瘤细胞增殖的影响,以及对鸡胚尿囊膜血管生成的抑制作用。方法采用MTT法检测片仔癀对体外培养肿瘤细胞(肝癌SMMC-7721细胞、胃癌SGC-7901细胞、宫颈癌HeLa细胞)的抗肿瘤作用;以鸡胚尿囊膜血管生成(CAM)实验,研究片仔癀对新生血管生成的抑制作用。结果片仔癀在0.2～1.0mg/mL对各肿瘤细胞的增殖有显著抑制作用,1.0mg/mL片仔癀对肝癌细胞增殖抑制率达到81.55%;片仔癀在0.1～1000μg/mL对鸡胚尿囊膜血管生成有显著抑制作用,给药鸡胚的新生血管数减少,血管变细,其中10μg/mL抑制率最高,一级血管抑制率为61.1%,二级血管抑制率为59.1%。结论在实验给药浓度下,片仔癀对受试肿瘤细胞的增殖具有较强抑制作用,对鸡胚尿囊膜血管生成有显著抑制作用。Objective To study the effect of Pien Tze Huang （PTH） on tumor proliferation in vitro and chorioallantoic membrane （CAM） angiogenesis. Methods The effect of PTH on the proliferation of cancer cells （SMMC-7721, SGC-7901, and HeLa） were evaluated by MTT assay. The CAM angiogenesis model was established to determine the inhibitive effect of PTH against angiogenesis. Results PTH had significant inhibitive effect on tumor cells proliferation at doses of 0.2--1.0 mg/mL. With the dose of 1.0 mg/mL, the inhibition ratio of PTH was 81.55% on hepatoma carcinoma cell SMMC-7721; PTH significantly affected chick embryonic angiogenesis in doses range of 0.1--1 000 μg/mL and induced a reduction in the capillary plexus of the CAM. The central and secondary capillaries were reduced by 61.1% and 59.1% respectively, at the dose of 10 μg/mL. Conclusion PTH showed significant inhibition effect on tumor proliferation and chorioallantoic membrane angiogenesis in the doses of this study.

关 键 词：片仔癀:抗肿瘤 抑制血管生成 鸡胚尿囊膜 MTT法 

分 类 号：R979.1[医药卫生—药品]