纳米银温敏喷雾凝胶的毒性试验研究  

Toxicity of temperature-sensitive spray gel containing silver nanoparticles

作　　者：陈美婉[1,2] 余思琴[1,3] 杨志文[1,3] 沈飞海[1] 黄芝瑛[1] 吴传斌[1,3] 

机构地区：[1]中山大学药学院,广东广州510006 [2]澳门大学中华医药研究院,澳门999078 [3]中山大学药物制剂工程研究开发中心,广东广州510006

出　　处：《药物评价研究》2011年第3期174-178,共5页Drug Evaluation Research

基　　金：广东省科技计划项目(2007B03070400)

摘　　要：目的为确定用于治疗妇女细菌性阴道炎的纳米银温敏喷雾凝胶的安全性,对其体内外毒性进行研究,为进一步研发提供依据。方法采用小鼠急性毒性上下法评价纳米银温敏喷雾凝胶的体内毒性。采用倒置显微镜观察细胞的生长状况(定性)和噻唑蓝(MTT)比色法(定量)检测不同质量浓度的纳米银温敏喷雾凝胶对小鼠成纤维细胞L-929的体外细胞毒性,并评价相对增殖率。结果纳米银温敏喷雾凝胶ig给药对小鼠半数致死量(LD50)>5.0g/kg,表明纳米银温敏喷雾凝胶的急性毒性较低。纳米银温敏喷雾凝胶质量浓度低于7.0μg/mL时,对细胞的生长无明显抑制作用,但随着剂量的加大和作用时间的延长,对细胞生长的抑制作用越来越明显,并有一定的剂量依赖趋势,同时细胞在形态学上也出现了一系列变化。结论纳米银温敏喷雾凝胶无明显毒性。Objective To evaluate the toxicity of temperature-sensitive spray gel containing silver nanoparticles which was used for the treatment of women bacterial vaginosis.Methods The acute toxicity study was conducted using main test of up-and-down procedure.The cytotoxicity of this agent on rat’s fibroblast L-929 cells was examined with MTT method.MTT assay was performed to observe the toxicity of temperature-sensitive spray gel containing silver nanoparticles in different dose levels and to evaluate the cell proliferation.The morphologic changes of the cells were observed by inverted microscope.Results The study demonstrated that LD50 value was above 5 000mg/kg,which showed low acute toxicity of this gel.No significant inhibitive effect on the growth of L-929 cells was observed from inverted microscopy when the concentration of gel was less than 7.0 μg/mL.With increasing dose levels of temperature-sensitive spray gel containing silver nanoparticles and longer interaction time between this agent and L-929 cells,the inhibitive effect on the growth of L-929 cells was increased and showed the tendency of dose dependent.Meanwhile,the morphologic changes of the cells were significant.Conclusion No significant toxicity in vivo and in vitro is found in temperature-sensitive spray gel containing silver nanoparticles.

关 键 词：纳米银温敏喷雾凝胶 上下法 MTT法 L-929细胞 毒性研究 

分 类 号：R965.3[医药卫生—药理学]