参附注射液对复苏后大鼠肺组织核转录因子κB及血浆肿瘤坏死因子α的影响  

Effect of Shenfu injection on expression of nuclear factor κB and level of tumor necrosis factor α after cardiopulmonary resuscitation in rats

作　　者：顾桂国[1,2] 林兆奋[1] 李文放[1] 杨兴易[1] 

机构地区：[1]上海长征医院急救科,上海200003 [2]上海浦东新区医疗事故处理办公室,上海200136

出　　处：《内科理论与实践》2011年第3期225-229,共5页Journal of Internal Medicine Concepts & Practice

摘　　要：目的:探讨参附注射液对心肺复苏后大鼠肺组织核转录因子κB(NF-κB)及血浆肿瘤坏死因子α(TNF-α)的影响。方法:90只Sprague Dawley大鼠随机分为假手术组、模型组、参附治疗组,采用窒息合并冰氯化钾致大鼠心跳骤停-心肺复苏模型,运用免疫组化方法观察复苏后肺组织细胞中NF-κB的蛋白表达,采用放射免疫法检测血浆TNF-α水平;通过光镜和电镜观察肺组织细胞结构的变化。结果:与假手术组比较,复苏后肺组织NF-κB表达及血浆TNF-α水平均明显增加(P<0.01),NF-κB及血浆TNF-α分别于复苏后12 h和24 h达到高峰,并持续到48 h。与模型组相比,参附治疗组各时相点NF-κB及TNF-α表达均明显下降(P<0.05)。光镜和电镜观察结果显示参附治疗组肺组织细胞结构受损明显减轻。结论:参附注射液可能通过抑制NF-κB活性及降低TNF-α水平,从而阻断NF-κB调控的炎症反应及细胞凋亡,对复苏后肺组织损伤起到防治作用。Objective To study the effect of Shenfu injection on expression of nuclear factor κB（NF-κB） and level of tumor necrosis factor α（TNF-α） after cardiopulmonary resuscitation（CPR） in rats.Methods Ninety Sprague Dawley rats were randomly divided into 3 groups： sham control group（n=30）,resuscitation model group（n=30）,and Shenfu injection treatment group（SF,n=30）.Cardiac arrest was induced by asphyxia and injection of ice-cold 0.5 mmol/L KCl and resuscitation was begun five minutes afterwards.NF-κB expression in lung tissues was determined by immunocytochemistry at 3,6,12,12,24,48 h after resuscitation.Levels of plasma TNF-α were assayed by radioimmunoassay（RIA）.Histological appearances of lung tissue were examined under light and electron microscope.Results Expression of NF-κB and level of plasma TNF-α increased significantly（P0.01） in model group when compared with sham control group,and reached the peak at 12 h and 24 h,respectively.Meanwhile,expression of NF-κB and level of TNF-α were significantly lower in SF group than that in model group（P0.05）.Histological examination showed that lung injury in SF group was significantly ameliorated when compared with model group.Conclusions Shenfu injection may down-regulate the expression of NF-κB and level of TNF-α after CPR,thereby blocking the modulation of inflammatory response and cell apoptosis induced by NF-κB and exerts a protective effect on lung injury seen after CPR.

关 键 词：心肺复苏 肺组织损伤 缺血再灌注 参附注射液 核转录因子ΚB 肿瘤坏死因子Α 

分 类 号：R285.5[医药卫生—中药学]