精氨酸加压素对脓毒症大鼠动脉压力反射的影响  

Effect of arginine vasopressin on arterial baroreflex in septic rats

作　　者：杨磊[1] 柴琛[1] 程东升[2] 刘颖璐[3] 乔玮[1] 鲁彦[2] 曹农[1] 

机构地区：[1]兰州大学第一医院普外一科,甘肃兰州730000 [2]中国人民解放军第一医院检验科,甘肃兰州730030 [3]山东大学医学院,山东济南250100

出　　处：《中国普通外科杂志》2011年第6期599-604,共6页China Journal of General Surgery

基　　金：国家自然科学基金资助项目(30700266);陕西省卫生厅资助项目(06E05);中央高校基本科研业务费专项资金(lzujbky-2010-146)

摘　　要：目的观察精氨酸加压素(AVP)通过中枢及外周给药对脓毒症大鼠动脉压力反射(ABR)的影响。方法采用盲肠结扎穿刺术(CLP)制作大鼠脓毒症模型。(1)分别于大鼠造模后4、8和12 h检测两组大鼠压力反射(ABR)功能以及下丘脑视上核(SON)和室旁核(PVN)精氨酸加压素(AVP)的表达,以假手术大鼠为对照;(2)大鼠造模后8 h侧脑室注射AVP(50 ng),以等体积的人工脑脊液(aCSF)为对照,于给药后5 min和30 min测ABR功能;(3)大鼠造模后6 h静脉持续泵入AVP[2 ng/(kg.h)],以等体积的生理盐水为对照,分别于给药后5 min和30 min测ABR功能。结果 CLP术后,大鼠ABR功能以及SON和PVN中的AVP阳性细胞表达随时间呈进行性降低和减少。侧脑室注射AVP或静脉持续输入AVP均能明显改善脓毒症大鼠的ABR功能,与各自的对照组相比差异有统计学意义(均P<0.05)。结论脓毒症发病过程中存在中枢AVP的减少和ABR功能降低,可能是脓毒症进展的关键因素。脓毒症早期给予外源性的AVP可改善大鼠的ABR,延缓脓毒症的进展。Objective To investigate the effects of central and peripheral injection of arginine vasopressin（AVP） on arterial baroreflex（ABR） function in septic rats.Methods Rat sepsis model was induced by cecal ligation and puncture（CLP）.（1） The ABR function and AVP expressions in supraoptic and paraventricular nucleus of the 2 grous were measured at 4,8 and 12 h after operation,respectively.The sham operated rats were used as control;（2） Sepsis rats were given AVP（50 ng）,and artificial cerebrospinal fluid of the same volume were given to control rats by intracerebroventricular injection 8 h after operation.The ABR functions of the 2 group rats were determined at 5 and 30 min after injection.（3）Sepsis rats were given AVP [2 ng/（kg·h）] and control rats were given saline of the same volume by continuous intravenous infusion 6 h after CLP.The ABR functions of the 2 group rats were determined at 5 and 30 min after infusion.Results In sepsis rats showed gradual decrease in ABR function,as well as a gradual reduction of AVP expressions in supraoptic and paraventricular nucleus.Both intracerebroventricular injection and continuous intravenous infusion with AVP significantly improved the ABR functions in sepsis rats compared with control group rats（both P〈0.05）.Conclusions In sepsis,the reduction of AVP expression in supraoptic and paraventricular nucleus induces a decrease in ABR function,which may probably contribute to the development of sepsis.Use of exogenous AVP in the initial phase of sepsis can improve ABR function and delay the development of sepsis.

关 键 词：脓毒症 精氨酸加压素 压力反射 大鼠 

分 类 号：R631[医药卫生—外科学]